Activation of hippocampal postsynaptic muscarinic receptors is involved in long-term spatial memory formation
Activation of hippocampal postsynaptic muscarinic receptors is involved in long-term spatial memory formation
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DOI：
10.1111/j.1460-9568.2007.05391.x
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发表时间：
2007-03-01
影响因子：
3.4
通讯作者：
Bermudez-Rattoni, Federico
中科院分区：
文献类型：
--
作者：
Herrera-Morales, Wendy;Mar, Ivonne;Bermudez-Rattoni, Federico
空间记忆与海马功能密切相关。有几个报告的参与，这一结构的收购和巩固的空间任务。在这项研究中，我们评估了选择性和非选择性的毒蕈碱拮抗剂在大鼠背侧海马在收购和编码的空间任务的影响。大鼠在Morris水迷宫中训练4天，每天训练相同的时间，并在训练后1周测试长期记忆（LTM）。在每天训练开始前20分钟，在背侧海马中双侧注射动物。结果表明，非选择性毒蕈碱拮抗剂东莨菪碱可阻断水迷宫记忆形成的获得。此外，微量注射选择性突触后毒蕈碱拮抗剂，哌仑西平，破坏LTM，而它不影响收购。相反，选择性突触前毒蕈碱拮抗剂，AFDX-116，没有破坏水迷宫收购或LTM形成。AFDX-116和哌仑西平的组合具有与东莨菪碱相似的作用，部分阻断了获取并损害了长期空间记忆。这些结果支持的观点，毒蕈碱受体参与空间学习和突触后毒蕈碱受体在背海马特别是参与长期空间记忆的形成。
Spatial memory has been strongly associated with hippocampal function. There are several reports of the participation of this structure in acquisition and consolidation of spatial tasks. In this study, we evaluated the effects of selective and non-selective muscarinic antagonists in the dorsal hippocampus of rats during acquisition and encoding of a spatial task. Rats were trained in a Morris water maze for 4 days with identical daily sessions, and tested for long-term memory (LTM) 1 week after training. The animals were injected bilaterally in the dorsal hippocampus 20 min before the start of every day of training. The results showed that the non-selective muscarinic antagonist, scopolamine, disrupted acquisition of water maze memory formation. Moreover, microinjections of a selective postsynaptic muscarinic antagonist, pirenzepine, disrupted LTM, whereas it did not affect acquisition. Conversely, a selective presynaptic muscarinic antagonist, AFDX-116, did not disrupt either water maze acquisition or LTM formation. Combination of AFDX-116 and pirenzepine had similar effects as scopolamine, partially blocking acquisition and impairing long-term spatial memory. These results support the view that muscarinic receptors are involved in spatial learning and that postsynaptic muscarinic receptors in the dorsal hippocampus are particularly involved in long-term spatial memory formation.