GP73, a resident Golgi glycoprotein, is sensibility and specificity for hepatocellular carcinoma of diagnosis in a hepatitis B-endemic Asian population
GP73, a resident Golgi glycoprotein, is sensibility and specificity for hepatocellular carcinoma of diagnosis in a hepatitis B-endemic Asian population
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DOI：
10.1007/s12032-009-9215-y
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发表时间：
2010-06-01
期刊：
影响因子：
3.4
通讯作者：
Miao, Xiong-yin
中科院分区：
文献类型：
--
作者：
Hu, Jin-song;Wu, De-wu;Miao, Xiong-yin
高尔基体蛋白73（Golgi protein-73，GP 73）是新近发现的肝癌候选血清标志物，但目前在亚洲人群中的研究较少。本研究的目的是通过Western blotting和RT-PCR方法检测正常人、B型肝炎、肝硬化和肝癌患者血清中GP 73的表达情况，并建立血清GP 73蛋白和RNA诊断肝癌的敏感性和特异性。采用免疫印迹法和RT-PCR法检测血清GP 73，光密度法定量。对124例不同类型肝脏患者血清中的GP 73进行了检测。使用市售的电化学发光免疫测定法检测AFP。HBV相关性肝癌患者sGP 73的中位数显著高于健康人和其他疾病患者（P < 0.001）。sGP 73蛋白检测HBV相关性肝癌的敏感性为77.4%，特异性为83.9%，最佳临界值为7.4个相对单位。受试者工作特征曲线下面积为0.89。GP 73 RNA检测HBV相关性HCC的敏感性为87.1%，特异性为83.9%，AUROC为0.92。AFP对HCC患者的敏感性为48.4%，特异性为96.8%，AUROC为0.77。亚洲HBV相关性肝癌患者血清中GP 73蛋白和RNA含量明显高于其他肝脏疾病。GP 73对HBV相关性肝癌的诊断优于AFP。RT-PCR是一种比Western blot更灵敏和更上级的定量方法。此外，我们的数据需要在更大的患者队列中得到证实。
Golgi protein-73 (GP73) is a newly identified candidate serum marker for HCC, but GP73 study now is lesser in Asian population. The aims of this study were to determine how GP73 is detected in the serum of healthy, hepatitis B, cirrhosis and HCC by western blotting and RT-PCR, and to establish the sensitivity and specificity of serum GP73 protein and RNA for diagnosing HCC. Serum GP73 was detected by western blotting and RT-PCR, and quantified by densitometric analysis. GP73 was measured in serum from 124 patients with various forms of liver. AFP was tested using commercially available electrochemiluminescence immunoassay. The median sGP73 in patients with HBV-related HCC was significantly higher (P < 0.001) than in healthy individuals and in patients with other diseases. When sGP73 protein was used to detect HBV-related HCC, it had a sensitivity of 77.4% and a specificity of 83.9%, at the optimal cut-off value of 7.4 relative units. The area under the receiver-operating characteristic curve was 0.89. GP73 RNA in patients with HBV-related HCC had a sensitivity of 87.1% and a specificity of 83.9% and AUROC of 0.92. AFP in patients with HCC had a sensitivity of 48.4% and a specificity of 96.8% and AUROC of 0.77. GP73 protein and RNA can be found in the serum of patients with HBV-related HCC obviously higher than of other liver diseases in Asian. GP73 was better than AFP for the diagnosis of HBV-related HCC. RT-PCR is a more sensitive and superior method of quantification than Western blot. Furthermore, our data need to be confirmed in larger cohorts of patients.