A cellular and molecular atlas reveals the basis of chytrid development.
A cellular and molecular atlas reveals the basis of chytrid development.
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细胞和分子图谱揭示了壶菌发育的基础。
DOI：
10.7554/elife.73933
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发表时间：
2022-03-01
期刊：
影响因子：
7.7
通讯作者：
Cunliffe M
中科院分区：
文献类型：
--
作者：
Laundon D;Chrismas N;Bird K;Thomas S;Mock T;Cunliffe M
壶菌（Chytridiomycota）是一个重要的真菌分支，具有重要的生态和进化意义。尽管它们的重要性，壶菌发育和细胞生物学的许多基本方面仍然知之甚少。为了解决这些知识的差距，我们结合定量体积电子显微镜和比较转录组分析创建一个'图集'的壶菌生命周期的细胞和分子基础，使用模型壶菌Rhizoclosmatium globosum。从我们的发育图谱中，我们描述了从转录不活跃的自由游动游动孢子到生物学上更复杂的胚芽的过渡，并表明脂质加工在整个生命周期中是多方面的和动态的。我们表明，壶菌突起是一个高的细胞内贩运，连接喂养/附着假根生殖游动孢子囊，并构成分工壶菌细胞计划的一个区室化的网站。我们提供的证据表明，在游动孢子发生过程中，游动孢子显示变形虫形态和表现出从间质母体细胞质的内吞货物运输。总之，我们的研究结果揭示了壶菌发育生物学的见解，并为未来的非二核体真菌细胞生物学研究提供了基础。
The chytrids (phylum Chytridiomycota) are a major fungal lineage of ecological and evolutionary importance. Despite their importance, many fundamental aspects of chytrid developmental and cell biology remain poorly understood. To address these knowledge gaps, we combined quantitative volume electron microscopy and comparative transcriptome profiling to create an ‘atlas’ of the cellular and molecular basis of the chytrid life cycle, using the model chytrid Rhizoclosmatium globosum. From our developmental atlas, we describe the transition from the transcriptionally inactive free-swimming zoospore to the more biologically complex germling, and show that lipid processing is multifaceted and dynamic throughout the life cycle. We demonstrate that the chytrid apophysis is a compartmentalised site of high intracellular trafficking, linking the feeding/attaching rhizoids to the reproductive zoosporangium, and constituting division of labour in the chytrid cell plan. We provide evidence that during zoosporogenesis, zoospores display amoeboid morphologies and exhibit endocytotic cargo transport from the interstitial maternal cytoplasm. Taken together, our results reveal insights into chytrid developmental biology and provide a basis for future investigations into non-dikaryan fungal cell biology.