Detection of Misfolded α-Synuclein Aggregates in Cerebrospinal Fluid by the Protein Misfolding Cyclic Amplification Platform
Detection of Misfolded α-Synuclein Aggregates in Cerebrospinal Fluid by the Protein Misfolding Cyclic Amplification Platform
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DOI：
10.1007/978-1-4939-9124-2_4
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发表时间：
2019-01-01
期刊：
影响因子：
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通讯作者：
Soto, Claudio
中科院分区：
文献类型：
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作者：
Concha-Marambio, Luis;Shahnawaz, Mohammad;Soto, Claudio
在此，我们描述了使用缓冲液中的α S合成聚集体建立α S-PMCA测定并准确检测人CSF样品中内源性α S聚集体的详细方案。鉴于该技术的扩增性质，在人体体液中循环的微量错误折叠蛋白质聚集体可以倍增，然后通过更常规的方法检测，如免疫测定或荧光。遵循这些原则，标准化α S-PMCA，用于高度灵敏和特异性检测突触核蛋白病患者脑脊液（CSF）中的α S错误折叠聚集体。
Here, we describe a detailed protocol to set up the alpha S-PMCA assay using alpha S synthetic aggregates in buffer and to accurately detect endogenous alpha S aggregates from human CSF samples. Given the amplificative nature of the technique, minute amounts of misfolded protein aggregates circulating in human bodily fluids can be multiplied and thereafter detected by more conventional methods, such as immune assays or fluorescence. Following these principles, alpha S-PMCA was standardized for the highly sensitive and specific detection of alpha S misfolded aggregates in cerebrospinal fluid (CSF) of patients with synucleinopathies.