Microbial degradation of fomesafen and detoxification of fomesafen-contaminated soil by the newly isolated strain Bacillus sp FE-1 via a proposed biochemical degradation pathway
Microbial degradation of fomesafen and detoxification of fomesafen-contaminated soil by the newly isolated strain Bacillus sp FE-1 via a proposed biochemical degradation pathway
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新分离菌株 Bacillus sp FE-1 通过拟议的生化降解途径对氟磺胺草醚进行微生物降解和对氟磺胺草醚污染土壤的解毒
DOI：
10.1016/j.scitotenv.2017.10.151
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发表时间：
2018-03-01
影响因子：
9.8
通讯作者：
Yu, Yunlong
中科院分区：
文献类型：
--
作者：
Cui, Ning;Wang, Saige;Yu, Yunlong
氟草胺是一种长期残留的除草剂，对环境安全构成潜在风险，因此越来越需要寻找生态友好且具有成本效益的技术来修复被氟草胺污染的土壤。本文从稻田土壤中分离到一株新的芽孢杆菌FE-1。发现该菌株在液体介质和土壤中都能降解塑料。在0.5、1和10 mg L-1的浓度下，芽孢杆菌FE-1在液体培养基中降解82.9%的氟磺胺，降解的最佳pH和温度分别为7.0和35℃。菌株FE-1接种后，土壤样品中腐殖酸降解率显著提高。检测并鉴定了两种芬美沙芬降解代谢物：氨基芬美沙芬和5-[2-氯-4-（三氟甲基）苯氧基]-2-氨基苯甲酸。这是第一次报道一种新的生物降解途径，涉及硝基的还原，然后是酰胺键的水解。对敏感后茬玉米的生物试验表明，FE-1对污染土壤具有良好的修复能力。结果表明，芽孢杆菌FE-1具有应用于腐殖酸污染土壤生物修复的潜力。(c) 2017 Elsevier B.V.版权所有
Fomesafen is a long residual herbicide and poses a potential risk to environmental safety, leading to an increasing need to find eco-friendly and cost-effective techniques to remediate fomesafen-contaminated soils. In this article, a novel strain of Bacillus sp., FE-1 was isolated from paddy field soil. This strain was found to degrade fomesafen both in liquid medium and in soil. >82.9% of fomesafen, at concentrations of 0.5, 1 and 10 mg L-1, was degraded by Bacillus sp. FE-1 in liquid medium within 14 h. The optimal pH and temperature for degradation were 7.0 and 35 degrees C, respectively. Soil samples inoculated with strain FE-1 showed significantly increased rates of fomesafen degradation. Two metabolites of fomesafen degradation were detected and identified as amino-fomesafen and 5-[2-chloro-4-(trifluoromethyl) phenoxy]-2-amino-benzoic acid. This is the first report of a novel fomesafen biodegradation pathway involving the reduction of a nitro group followed by the hydrolysis of an amide bond. The excellent remediation capability of the isolate FE-1 to detoxify fomesafen-contaminated soil was shown by bio-assay of the sensitive aftercrop corn. The results indicate that Bacillus sp. FE-1 has potential for use in the bioremediation of fomesafen-contaminated soil. (c) 2017 Elsevier B.V. All rights reserved.