The influence of HCMV infection on autophagy in THP-1 cells.
The influence of HCMV infection on autophagy in THP-1 cells.
复制标题
HCMV感染对THP-1细胞自噬的影响
DOI：
10.1097/md.0000000000008298
复制
发表时间：
2017-11
期刊：
影响因子：
1.6
通讯作者：
Shang S
中科院分区：
文献类型：
--
作者：
Liu Y;Pan J;Liu L;Li W;Tao R;Chen Y;Li H;Shang S
背景：人类巨细胞病毒（HCMV）感染是非常常见的，潜伏期可以在未来重新激活。它可以改变细胞内环境，类似于其他疱疹病毒，以促进病毒的复制和生存。本研究旨在探讨HCMV感染对人急性单核细胞白血病细胞系（THP-1细胞）自噬的影响。方法：采用逆转录聚合酶链反应（RT-PCR）、免疫印迹（western blot）和透射电镜（TEM）检测小鼠自噬水平。计算自噬相关蛋白Beclin 1、Atg5和轻链3 （LC3）的浓度，并与actin水平进行比较。结果：与对照THP-1细胞相比，感染后1 ~ 5天Beclin1、Atg5和LC3II mrna的表达水平逐渐升高，随后逐渐下降。然而，western blot分析结果显示，LC3II水平在感染后1天逐渐升高，感染后7天下降。而Atg5和Beclin1分别在感染后第2天逐渐升高，第5天开始下降。结论：HCMV感染可促进细胞自噬，潜伏期细胞自噬水平降低。需要进一步研究HCMV潜伏期与自噬之间的关系，以确定自噬在HCMV潜伏感染中的作用，从而为临床治疗寻找治疗方法。
Background: Human cytomegalovirus (HCMV) infection is very common and latency can be reactivated in the future. And it can alter the intracellular environment, similar to other herpesviruses, for viral replication and survival. The aim of this study was to investigate the influence of HCMV infection on autophagy in human acute monocytic leukemia cell line (THP-1 cells). Methods: Reverse transcription polymerase chain reaction (RT-PCR), western blot, and transmission electron microscope (TEM) were used to examine autophagy level. The concentrations of autophagy-related proteins Beclin 1, Atg5, and the light chain three (LC3) were counted when compared with actin level. Results: The expression levels of Beclin1, Atg5, and LC3II mRNAs increased gradually between 1 and 5 days(d) postinfection (p.i.) and subsequently decreased little by little when compared with the control THP-1 cells. However, results of western blot analysis displayed that the level of LC3II increased gradually after 1 day p.i. and decreased at 7 days after infection. But the levels of Atg5 and Beclin1 increased gradually after 2 days p.i. and began to decrease at 5 days after infection, respectively. Conclusion: These results suggested that HCMV infection can facilitate the autophagy and autophagy level may decrease in latent phase. More studies on the relationship between HCMV latency and autophagy are needed to determine the role of autophagy in HCMV latent infection that may help find out a therapeutic approach for clinical treatment.