A Novel Platelet-Repellent Polyphenolic Surface and Its Micropattern for Platelet Adhesion Detection.
A Novel Platelet-Repellent Polyphenolic Surface and Its Micropattern for Platelet Adhesion Detection.
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DOI：
10.1021/acsami.6b08930
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发表时间：
2016-09
影响因子：
9.5
通讯作者：
Liwei Yang;Lulu Han;Lingyun Jia
中科院分区：
文献类型：
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作者：
Liwei Yang;Lulu Han;Lingyun Jia
表面图案化是诊断血小板粘附的有力工具。然而，目前构建血小板图案表面的方法需要费力和复杂的步骤。本文报道了一种新的简单的血小板排斥表面，即金属（Fe3+离子）-多酚（单宁酸，TA）配位相互作用。长期驱血小板效果明显优于聚乙二醇（PEG）。此外，血小板排斥行为可以扩展到其他多酚功能化表面。在此基础上，通过一步微接触印刷原位制备了基于ta的微图案，该微图案具有良好的血小板粘附性，可以有效地避免传统的惰性亲水性聚合物和生物活性配体的引入。随后，以ta为基础的微模式应用于监测抗血小板药物（替罗非班）治疗的缺陷血小板的粘附。这项工作提供了一种简单、通用和环保的策略来构建血小板排斥多酚表面及其微图案。我们期望这种简单的微模式可以作为低成本和无标签的生物材料和生物传感器平台，并可广泛用于血小板粘附功能的临床诊断和抗血小板治疗的评估。
Surface patterning provides a powerful tool to the diagnosis of platelet adhesion. However, the current methodologies of constructing platelet-patterned surfaces require laborious and complicated steps. Herein, a novel and simple platelet-repellent surface was reported by metal (Fe3+ ions)-polyphenol (tannic acid, TA) coordination interaction. The platelet-repellent effect was significantly better than that of poly(ethylene glycol) (PEG) in a long-term. Moreover, the platelet-repellent behavior could extend to other polyphenols-functionalized surfaces. On the basis of these observations, a TA-based micropattern was fabricated in situ by one-step microcontact printing for well-defined platelet adhesion, which can effectively avoid the traditional introduction of inert hydrophilic polymers and bioactive ligands. Afterward, the TA-based micropattern was applied to monitor the adhesion of defective platelets treated with an antiplatelet drug (tirofiban). This work provided a facile, versatile, and environmentally friendly strategy to construct platelet-repellent polyphenolic surfaces and their micropattern. We expect that this simple micropattern could act as a low-cost and label-free platform for biomaterials and biosensors, and could be widely used in the clinical diagnoses of platelet adhesive functions and the evaluation of antiplatelet therapies.