FGF-8 is associated with anteroposterior patterning and limb regeneration in Xenopus
FGF-8 is associated with anteroposterior patterning and limb regeneration in Xenopus
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DOI：
10.1006/dbio.1997.8732
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发表时间：
1997-12-15
影响因子：
2.7
通讯作者：
Slack, JMW
中科院分区：
文献类型：
--
作者：
Christen, B;Slack, JMW
尽管它在鸡和小鼠的肢体发育中也有一些较早的表达结构域，但由于它对小鸡和小鼠肢体发育的重要性，它引起了人们的注意。我们现在已经从非洲爪哇克隆了一段成纤维细胞生长因子-8的同源物，这样就更容易对早期发育进行功能研究。没有母体表达，合子表达在原肠胚期和神经胚期最高。XFGF-8的表达形式是围绕胚孔的一个环，随后在尾芽中表达。头部有几个区域，包括孵化腺、鳃裂隙和中脑-后脑边界。在发育后期，肢芽表皮有一条明显的表达带。虽然没有形态上的顶脊，但这条表达带表明非洲爪哇的肢芽包含一个隐蔽的区域，具有类似的刺激间充质生长的能力。XFGF8的中胚层诱导活性略低于其他FGFs，而后胚化活性与其他FGFs相似。这些差异可能是由于不同的受体特异性所致。XFGF8的后部表达和高的后部化活性表明，XFGF8在原肠发育过程中参与了成纤维细胞生长因子家族成员的前后轴的形成。与羊膜不同的是，非洲爪哇的肢芽在受损后可以再生。我们发现再生与XFGF-8在远端表皮中的重新表达相关，这表明这种能力对于成功的肢体再生是至关重要的。(C)1997年学术出版社。
FGF-8 has attracted attention particularly because of its importance for limb development in the chick and mouse, although it also has a number of earlier expression domains in these species. We have now cloned an FGF-8 homologue from Xenopus in which it is easier to do functional studies on early development. There is no maternal expression, while zygotic expression is highest in the gastrula and neurula stages. XFGF-8 is expressed as a ring around the blastopore and subsequently in the tail bud. There are several domains in the head including the hatching gland, the branchial clefts, and the midbrain-hindbrain border. At later stages there is a prominent band of expression in the limb bud epidermis. Although there is no morphological apical ridge, this band of expression suggests that the Xenopus limb bud contains a cryptic region with a similar ability to stimulate mesenchymal outgrowth. The mesoderm-inducing activity of XFGF-8 is somewhat lower than that of other FGFs, while the posteriorizing activity is similar. These differences are probably due to the different receptor specificity. The posterior expression and high posteriorizing activity suggest that XFGF-8 contributes to the patterning of the anterior-posterior axis by FGF family members during gastrulation. In contrast to the amniotes, Xenopus limb buds can regenerate following damage. We show that regeneration is correlated with the reexpression of XFGF-8 in the distal epidermis, suggesting that this ability is critical for successful limb regeneration. (C) 1997 Academic Press.