An immunostimulatory DNA sequence from a probiotic strain of Bifidobacterium longum inhibits IgE production in vitro
An immunostimulatory DNA sequence from a probiotic strain of Bifidobacterium longum inhibits IgE production in vitro
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DOI：
10.1111/j.1574-695x.2006.00064.x
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发表时间：
2006-04-01
影响因子：
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通讯作者：
Saito, T
中科院分区：
文献类型：
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作者：
Takahashi, N;Kitazawa, H;Saito, T
从益生菌长双歧杆菌BB 536的基因组DNA中鉴定出免疫刺激性寡脱氧核苷酸（ODN）BL 07（5 '-GCGTCGGTTTCGGTGCTCAC-3'）。ODN BL 07刺激巨噬细胞样J774.1细胞中B淋巴细胞增殖并诱导白细胞介素-12（IL-12）的产生。ODNs BL 07和BL 07 S（修饰有硫代磷酸酯骨架）显著抑制免疫球蛋白E（IgE）的产生并刺激干扰素-γ（IFN-γ）和IL-12的产生，但不影响卵清蛋白致敏的BALB/c小鼠的小鼠脾细胞中IL-4的分泌。这些ODNs在IL-4和抗-CD 40存在下也显著抑制纯化的鼠B细胞中IgE的产生。结果提示ODNs BL 0 7和BL 0 7 S在预防IgE相关免疫应答中的潜力以及ODNs BL 0 7可能参与B的抗过敏功效。longum BB536.
The immunostimulatory oligodeoxynucleotide (ODN) BL07 (5'-GCGTCGGTTTCGGTGCTCAC-3') was identified from the genomic DNA of the probiotic strain Bifidobacterium longum BB536. ODN BL07 stimulated B-lymphocyte proliferation and induced interleukin-12 (IL-12) production in macrophage-like J774.1 cells. ODNs BL07 and BL07S (modified with phosphorothioate backbone) significantly inhibited immunoglobulin E (IgE) production and stimulated interferon-gamma (IFN-gamma) and IL-12 production, but did not affect IL-4 secretion in murine splenic cells of ovalbumin-primed BALB/c mice. These ODNs also significantly inhibited production of IgE in purified murine B cells in the presence of IL-4 and anti-CD40. The results suggest the potential of ODNs BL07 and BL07S in preventing IgE-related immune responses and the possible involvement of ODN BL07 in the antiallergic efficacy of B. longum BB536.