Nucleotide sequence analysis of Vietnamese highly pathogenic porcine reproductive and respiratory syndrome virus from 2013 to 2014 based on the NSP2 and ORF5 coding regions
Nucleotide sequence analysis of Vietnamese highly pathogenic porcine reproductive and respiratory syndrome virus from 2013 to 2014 based on the NSP2 and ORF5 coding regions
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DOI：
10.1007/s00705-015-2699-1
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发表时间：
2016-03-01
影响因子：
2.7
通讯作者：
Chae, Chanhee
中科院分区：
文献类型：
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作者：
Duy Tien Do;Park, Changhoon;Chae, Chanhee
对2013-2014年从越南分离的34株高致病性猪繁殖与呼吸综合征病毒（HP-PRRSV）进行了序列测定和分析。使用对应于非结构蛋白2（Nsp 2）编码区的ORF 1a的部分序列和开放阅读框5（ORF 5）基因的全序列进行分析。从从未接种过PRRSV疫苗的猪群中分离出HP-PRRSV毒株。与非结构蛋白2（Nsp 2）编码区和ORF 5对应的ORF 1a部分的核苷酸序列同一性分别为96.4%至100%和83.2%至100%。所有34株越南HP-PRRSV株均在Nsp 2编码区存在两个不连续的30个氨基酸缺失，可作为中国HP-PRRSV原型的遗传标记。34株越南HP-PRRSV分离株中有29株的ORF 5第13位和第151位存在精氨酸（R）氨基酸，中国HP-PRRSV原型株也存在精氨酸（R）氨基酸。所有越南HP-PRRSVs的ORF 5基因的序列分析揭示了六个亚组：Viet 1至4，JAX 1样和VR-2332样。2013-2014年34株越南HP-PRRSV分离株的核苷酸和氨基酸序列分析表明，与2012年之前分离的越南HP-PRRSV相比，近年来越南HP-PRRSV经历了快速的进化变化。
A total of 34 highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) strains isolated from Vietnam during 2013-2014 were sequenced and analyzed. A partial sequence of ORF1a corresponding to the nonstructural protein 2 (Nsp2) coding region and the full sequence of open reading frame 5 (ORF5) gene was used for the analysis. The HP-PRRSV strains were isolated from pig herds that had never been vaccinated for PRRSV. Nucleotide sequence identities in the portions of ORF1a corresponding to the nonstructural protein 2 (Nsp2) coding region and ORF5 ranged from 96.4 to 100 % and 83.2 to 100 %, respectively. All of the 34 Vietnamese HP-PRRSV strains showed two discontinuous 30-amino-acid deletions in the Nsp2 coding region as a genetic marker of prototypic Chinese HP-PRRSV. The amino acid arginine (R) was present at positions 13 and 151 in ORF5 in 29 out of 34 Vietnamese HP-PRRSV isolates, as well as in the prototypic Chinese HP-PRRSV. Sequence analysis of the ORF5 genes of all Vietnamese HP-PRRSVs revealed six subgroups: Viet 1 to 4, JAX1-like, and VR-2332-like. Nucleotide and amino acid sequence analysis of 34 Vietnamese HP-PRRSV isolates from 2013-2014 indicated that Vietnamese HP-PRRSV has undergone rapid evolutionary changes in recent years when compared with Vietnamese HP-PRRSV isolated before 2012.