Mucin-chitosan complexes at the solid-liquid interface: Multilayer formation and stability in surfactant solutions
Mucin-chitosan complexes at the solid-liquid interface: Multilayer formation and stability in surfactant solutions
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DOI：
10.1021/la0511844
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发表时间：
2005-10-11
期刊：
影响因子：
3.9
通讯作者：
Auletta, T
中科院分区：
文献类型：
--
作者：
Dedinaite, A;Lundin, M;Auletta, T
利用椭圆偏振法、干涉表面仪和原子力显微镜技术，研究了一种具有重要生物学意义的糖蛋白、粘蛋白和粘蛋白-壳聚糖复合物层在负电荷表面二氧化硅和云母上的吸附。特别注意阴离子表面活性剂十二烷基硫酸钠（SDS）对吸附层稳定性的影响。已经证明粘蛋白吸附在带负电荷的表面上，形成高度水合的层。这种粘蛋白层很容易与表面活性剂结合，并且很容易通过浓度为>= 0.2 cmc的SDS溶液冲洗从表面去除（1 cmc SDS在30 mM NaCl中等于3.3 mM）。粘蛋白吸附层带负电荷，我们展示了带正电荷的聚电解质壳聚糖如何与预吸附的粘蛋白结合，形成粘蛋白-壳聚糖复合物，即使在SDS浓度高达1cmc时也能抵抗SDS的解吸。因此，提供了一种防止表面活性剂去除粘蛋白层的方法。此外，我们展示了如何形成黏液-壳聚糖多层膜。
The adsorption of a biologically important glycoprotein, mucin, and mucin-chitosan complex layer formation on negatively charged surfaces, silica and mica, have been investigated employing ellipsometry, the interferometric surface apparatus, and atomic force microscopy techniques. Particular attention has been paid to the effect of an anionic surfactant sodium, dodecyl sulfate (SDS), with respect to the stability of the adsorption layers. It has been shown that mucin adsorbs on negatively charged surfaces to form highly hydrated layers. Such mucin layers readily associate with surfactants and are easily removed from the surfaces by rinsing with solutions of SDS at concentrations >= 0.2 cmc (1 cmc SDS in 30 mM NaCl is equal to 3.3 mM). The mucin adsorption layer is negatively charged, and we show how a positively charged polyelectrolyte, chitosan, associates with the preadsorbed mucin to form mucin-chitosan complexes that resist desorption by SDS even at SDS concentrations as high as 1 cmc. Thus, a method of mucin layer protection against removal by surfactants is offered. Further, we show how mucin-chitosan multilayers can be formed.