Novel lncRNA LINC00941 Promotes Proliferation and Invasion of Colon Cancer Through Activation of MYC.
Novel lncRNA LINC00941 Promotes Proliferation and Invasion of Colon Cancer Through Activation of MYC.
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DOI：
10.2147/ott.s293519
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发表时间：
2021
影响因子：
4
通讯作者：
Luo S
中科院分区：
文献类型：
--
作者：
Chang L;Zhou D;Luo S
我们进行了这项研究，以阐明LncRNA LINC 00941如何影响结肠癌的进展及其可能的调控机制。采用qRT-PCR检测LINC 00941在结肠癌组织和细胞中的表达水平。CCK-8法和Transwell法检测LINC 00941对结肠癌细胞增殖、迁移和侵袭的影响。LINC 00941、miR-205- 5 p和MYC之间的靶标相互作用通过双荧光素酶报告基因测定和RNA下拉实验进一步证实。同时，进行体内实验以研究LINC 00941在异种移植模型中的作用。LINC 00941在结肠癌组织和细胞中的表达水平升高。LINC 00941过表达加速了结肠癌细胞的增殖、迁移和侵袭，而LINC 00941敲低则显示了相反的结果。此外，LINC 00941通过海绵化miR-205- 5 p作为竞争性内源RNA来调节MYC的表达，并且miR-205- 5 p敲低逆转了LINC 00941敲低对结肠癌细胞的肿瘤抑制。异种移植模型试验证实LINC 00941沉默可抑制结肠癌细胞的生长和转移。LINC 00941可能通过作为ceRNA作用于miR-205- 5 p/MYC轴而显著促进结肠癌的进展，这为lncRNA指导结肠癌的治疗和预后提供了新的线索。
We conducted the study to elucidate how LncRNA LINC00941 affects colon cancer progression and its possible regulatory mechanism. The expression level of LINC00941 in colon cancer tissues and cells was detected by qRT-PCR. The function of LINC00941 on colon cancer cell proliferation, migration, and invasion was detected by CCK-8 and Transwell assay respectively. The target interactions among LINC00941, miR-205-5p, and MYC were further confirmed by dual-luciferase reporter gene assays and RNA pull-down experiments. Meanwhile, in vivo experiments were carried out to study the role of LINC00941 in the xenotransplantation model. LINC00941 expression level was elevated in colon cancer tissues and cells. LINC00941 overexpression accelerated proliferation, migration, and invasion of colon cancer cells, while the LINC00941 knockdown showed the opposite results. In addition, LINC00941 regulated the expression of MYC by sponging miR-205-5p as a competitive endogenous RNA, and miR-205-5p knockdown reversed the tumor inhibition of LINC00941 knockdown on colon cancer cells. Xenograft model assay confirmed that LINC00941 silencing could inhibit colon cancer cell growth and metastasis. LINC00941 may markedly promote colon cancer progression by acting on the miR-205-5p/MYC axis as a ceRNA, which offers novel clues for lncRNA to guide the treatment and prognosis of colon cancer.