Determination of imipenem and rifampicin in mouse plasma by high performance liquid chromatography-diode array detection
Determination of imipenem and rifampicin in mouse plasma by high performance liquid chromatography-diode array detection
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DOI：
10.1016/j.aca.2007.12.026
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发表时间：
2008-02-11
影响因子：
6.2
通讯作者：
Jimenez-Sanchez, J. C.
中科院分区：
文献类型：
--
作者：
Fernandez-Torres, R.;Bello-Lopez, M. A.;Jimenez-Sanchez, J. C.
建立了测定亚胺培南和利福平的高效液相色谱法。该方法包括用甲醇进行血浆脱蛋白，在RP-18柱上进行梯度洗脱和二极管阵列检测。使用由甲醇和0.2 M硼酸盐缓冲液（pH 7.2）组成的移动的相在8分钟内进行分离。亚胺培南和利福平分别在300 nm和255 nm处检测。亚胺培南在0.3 - 30 μ g mL（-1）和利福平在1.5 - 20 μ g mL（-1）的血浆浓度范围内观察到线性响应。亚胺培南和利福平的检出限分别为0.07 μ g mL（-1）和0.47 μ g mL（-1）。(c)2007 Elsevier B. V.保留所有权利。
A high performance liquid chromatographic (HPLC) method for the determination of imipenem and rifampicin was developed and validated. The method involves plasma deproteinisation with methanol, gradient elution on a RP-18 column and diode array detection. Separation was carried out in 8 min using a mobile phase composed of methanol and 0.2 M borate buffer (pH 7.2). Imipenem and rifampicin were detected at 300 nm and 255 nm, respectively A linear response was observed at plasma levels ranged between 0.3 and 30 mu g mL(-1) for imipenem and 1.5 and 20 mu g mL(-1) for rifampicin. The detection limits were 0.07 mu g mL(-1) and 0.47 mu g mL(-1) for imipenem and rifampicin, respectively The method was applied to the determination of both compounds in mouse plasma samples. (c) 2007 Elsevier B.V. All rights reserved.