Improvement of culture conditions and evidence for nuclear transformation by homologous recombination in a red alga, cyanidioschyzon merolae 10D
Improvement of culture conditions and evidence for nuclear transformation by homologous recombination in a red alga, cyanidioschyzon merolae 10D
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DOI：
10.1093/pcp/pch087
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发表时间：
2004-06-01
影响因子：
4.9
通讯作者：
Tanaka, K
中科院分区：
文献类型：
--
作者：
Minoda, A;Sakagami, R;Tanaka, K
尽管最近测定了单细胞红藻蓝藻10D的核基因组序列，但作为一种重要的模式植物系统的DNA转化技术迄今尚未问世。在本研究中，通过对培养条件的改进，得到了较快的菌落生长速度(倍增时间为9.2 h)，并在结冷胶平板上形成了菌落。利用这些条件，分离出了UMP合成酶基因缺失的自发突变株(5-氟甲酸抗性)。然后，通过将野生型UMP合成酶基因导入细胞中来修复损伤，这表明DNA通过同源重组转化。
Although the nuclear genome sequence of Cyanidioschyzon merolae 10D, a unicellular red alga, was recently determined, DNA transformation technology that is important as a model plant system has never been available thus far. In this study, improved culture conditions resulted in a faster growth rate of C. merolae in liquid medium (doubling time = 9.2 h), and colony formation on gellan gum plates. Using these conditions, spontaneous mutants (5-fluoroortic acid resistant) deficient in the UMP synthase gene were isolated. The lesions were then restored by introducing the wild-type UMP synthase gene into the cells suggesting DNA transformation by homologous recombination.