ROLE OF MICROTUBULES IN THE MOVEMENT OF THE VEGETATIVE NUCLEUS AND GENERATIVE CELL IN TOBACCO POLLEN TUBES
ROLE OF MICROTUBULES IN THE MOVEMENT OF THE VEGETATIVE NUCLEUS AND GENERATIVE CELL IN TOBACCO POLLEN TUBES
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DOI：
10.1007/bf00230890
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发表时间：
1995-03-01
影响因子：
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通讯作者：
RAUDASKOSKI, M
中科院分区：
文献类型：
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作者：
ASTROM, H;SORRI, O;RAUDASKOSKI, M
抗微管药物谷氮灵处理烟草和有翼烟草的花粉粒，显著延缓了营养核(VN)和生殖细胞(GC)从粒到管端的运动，但对花粉管的伸长无明显影响。在烟草花粉管中，分别只有11%和48%的花粉管中的VN和GC主要位于花粉管的中部。在相应的对照材料中，79%和99%的花粉管在顶端附近含有VN和GC。间接免疫荧光显微镜和相关研究显示，在谷氨酰胺存在下生长的小管完全没有微管(MTS)，但微丝(MFS)明显完整。这些结果表明，当没有MTS而只有MFS时，VN和GC从颗粒进入管内是可能的，但移动很慢。在对照管中，MT束的平行取向和VN的延伸被解释为VN依赖于MT的运动所需的结构组织。
The germination and growth of pollen grains of Nicotiana tabacum and N. alata with the anti-microtubule drug oryzalin retarded significantly the movement of the vegetative nucleus (VN) and the generative cell (GC) from the grain to the tube apex but had no effect on pollen tube elongation. In N. tabacum, only 11% and 48% of the pollen tubes treated with oryzalin for 6 h and 12 h, respectively, had the VN and GC in the tube mainly in its middle part. In corresponding control materials, 79% and 99% of pollen tubes contained the VN and GC close to the apex. Indirect immunofluorescence microscopy and related studies of the tubes grown in the presence of oryzalin revealed complete absence of microtubules (MTs) but apparently intact microfilaments (MFs). These results suggested that the movement of VN and GC from the grain into the tube is possible when no MTs but only MFs are present, but the movement is then slow. In control tubes, the parallel orientation of MT bundles and extensions of VN were interpreted to represent the structural organization needed for the MT-dependent movement of VN.