Receptor editing in a transgenic mouse model: Site, efficiency, and role in B cell tolerance and antibody diversification
Receptor editing in a transgenic mouse model: Site, efficiency, and role in B cell tolerance and antibody diversification
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DOI：
10.1016/s1074-7613(00)80395-7
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发表时间：
1997-12-01
期刊：
影响因子：
32.4
通讯作者：
Rajewsky, K
中科院分区：
文献类型：
--
作者：
Pelanda, R;Schwers, S;Rajewsky, K
在其IgH和Ig kappa基因座中携带转基因重排V区基因编码自身反应特异性的小鼠将新出现的自身反应性祖细胞引导到b细胞前室，其中它们的受体通过二次V kappa- j kappa重排和RS重组进行编辑。编辑是一个有效的过程，因为突变小鼠产生正常数量的B细胞。在类似的非自反应性转基因菌株中，既没有看到前b细胞区室，也没有看到受体编辑。因此，前b细胞区室可能已经进化到编辑自身反应性细胞的受体，后来通过前b细胞受体的发明，通常被用于有效的抗体多样化，模仿自身反应性抗体，将大量祖细胞引导到该区室。
Mice carrying transgenic rearranged V region genes in their IgH and Ig kappa loci to encode an autoreactive specificity direct the emerging autoreactive progenitors into a pre-B cell compartment, in which their receptors are edited by secondary V kappa-J kappa rearrangements and RS recombination. Editing is an efficient process, because the mutant mice generate normal numbers of B cells. In a similar nonautoreactive transgenic strain, neither a pre-B cell compartment nor receptor editing was seen. Thus, the pre-B cell compartment may have evolved to edit the receptors of autoreactive cells and later been generally exploited for efficient antibody diversification through the invention of the pre-B cell receptor, mimicking an autoreactive antibody to direct the bulk of the progenitors into that compartment.