Fluorescent labeling analysis and electron probe microanalysis for alveolar ridge augmentation using calcium phosphate cement
Fluorescent labeling analysis and electron probe microanalysis for alveolar ridge augmentation using calcium phosphate cement
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DOI：
10.4012/dmj.21.296
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发表时间：
2002-12-01
影响因子：
2.5
通讯作者：
Chow, LC
中科院分区：
文献类型：
--
作者：
Fujikawa, K;Sugawara, A;Chow, LC
我们先前的组织病理学研究表明，由磷酸钙骨水泥（CPC）与水以5 g/mL的粉液比混合制成的增强块，放置在牙槽骨嵴上，逐渐被天然骨取代。在本研究中，荧光标记分析（弗拉）和电子探针显微分析（EPMA）进行了上述组织病理学研究的同一手术部位。在处死前1周结束的3周期间，将荧光标记剂（将掺入新形成的矿化组织中）每周两次肌内注射到犬中。从块中获得的标本进行弗拉评估新骨形成的程度和EPMA测量元素（Ca，P，Mg）的分布。弗拉结果显示术后1个月存在新形成的骨。EPMA结果显示，术后6个月，增强部位的元素分布与残留骨区域的元素分布相似。弗拉和EPMA检查也表明，种植体被自然骨包围和固定。CPC增强块对于牙槽嵴增强和骨整合种植体固定明显有用。
Our previous histopathological study showed that the augmentation block, prepared from a calcium phosphate cement (CPC) mixed with H2O at powder to liquid ratio of 5 g/mL, placed on the alveolar bone ridge, was gradually replaced by natural bone. In the present study, fluorescent labeling analysis (FLA) and electron probe microanalysis (EPMA) were performed on the same surgical site of the above histopathological study. Fluorescent labeling agents, that would be incorporated into newly formed mineralized tissues, were injected into dogs intramuscularly twice a week during the 3 week period that ended I week before sacrifice. The specimens obtained from the block were subjected to FLA for assessing the extent of new bone formation and to EPMA for measuring the elemental (Ca, P, Mg) distributions. FLA results showed the presence of newly formed bone at 1 month after surgery. EPMA results showed that the elemental distributions in the augmentation site were similar to those of the residual bone area at 6 months after surgery. FLA and EPMA examinations also indicated that the implants were surrounded and fixed by natural bone chronologically. A CPC augmentation block is clearly useful for alveolar ridge augmentation and osteointegrated implant fixation.