Lipid profile of cerebrospinal fluid in multiple sclerosis patients: a potential tool for diagnosis
Lipid profile of cerebrospinal fluid in multiple sclerosis patients: a potential tool for diagnosis
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DOI：
10.1038/s41598-019-47906-x
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发表时间：
2019-08-05
影响因子：
4.6
通讯作者：
Brieva, L.
中科院分区：
文献类型：
--
作者：
Nogueras, L.;Gonzalo, H.;Brieva, L.
多发性硬化(MS)是一种复杂的多因素神经病理。虽然其病因尚不清楚，但已证明免疫系统攻击髓鞘，导致脱髓鞘和轴突损伤。在MS和其他脱髓鞘疾病中，脂类作为髓鞘的主要成分之一已被推测参与其中。然而，在疾病过程中是否存在特定的改变模式仍然是一个争论的问题。在这里，我们使用脂质组学方法证明，在诊断时，MS患者的脑脊液呈现出155种不同的脂质种类，其中47种被鉴定。根据25种脂类的存在对MS患者进行初始分级聚类。当应用一种有监督的方法来改进这种分类时，获得了一个脂体特征。这个特征由15个分子组成，分别属于五个不同的脂类家族，包括脂肪酸(FAs)。以FA为靶点的方法揭示了该家族中的两个成员的差异：18：3n3和20：0(花生酸)。这些结果揭示了MS患者在诊断时的脑脊液脂肪组特征，这可能被认为是一种潜在的诊断工具。
Multiple sclerosis (MS) is a complex multifactorial neuropathology. Although its etiology remains unclear, it has been demonstrated that the immune system attacks myelin, leading to demyelination and axonal damage. The involvement of lipids as one of the main components of myelin sheaths in MS and other demyelinating diseases has been postulated. However, it is still a matter of debate whether specific alteration patterns exist over the disease course. Here, using a lipidomic approach, we demonstrated that, at the time of diagnosis, the cerebrospinal fluid of MS patients presented differences in 155 lipid species, 47 of which were identified. An initial hierarchical clusterization was used to classify MS patients based on the presence of 25 lipids. When a supervised method was applied in order to refine this classification, a lipidomic signature was obtained. This signature was composed of 15 molecules belonging to five different lipid families including fatty acids (FAs). An FA-targeted approach revealed differences in two members of this family: 18:3n3 and 20:0 (arachidic acid). These results reveal a CSF lipidomic signature in MS patients at the time of diagnosis that might be considered as a potential diagnostic tool.