Determination of the half-life of circulating leptin in the mouse.
Determination of the half-life of circulating leptin in the mouse.
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DOI：
10.1038/ijo.2016.238
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发表时间：
2017-03
期刊：
影响因子：
--
通讯作者：
Leibel RL
中科院分区：
文献类型：
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作者：
Burnett LC;Skowronski AA;Rausch R;LeDuc CA;Leibel RL
脂肪因子激素，瘦素，是体重稳态的主要成分。已经进行了大量的研究，施用重组小鼠瘦素作为实验试剂;然而，尚未仔细评估外源性施用重组小鼠瘦素后循环瘦素的半衰期。将外源性瘦素给予（3 mg瘦素/kg体重）十周龄禁食非肥胖雄性小鼠，并在七个时间点连续收集血浆;通过ELISA测量每个时间点的血浆瘦素浓度以估计小鼠瘦素的循环半衰期。在测试的生理环境下，小鼠瘦素的半衰期为40.2（+/-2.2）分钟。外源性瘦素给药后1小时的循环瘦素浓度是空腹时内源性瘦素浓度的170倍。小鼠瘦素的半衰期测定为40.2分钟。这些结果应该是有用的计划和解释实验采用外源性瘦素。广泛使用的外源性瘦素给药方案导致的循环瘦素的非生理性升高可能会混淆有关激素临床生物学某些方面的推论。
The adipokine hormone, leptin, is a major component of body weight homeostasis. Numerous studies have been performed administering recombinant mouse leptin as an experimental reagent; however, the half life of circulating leptin following exogenous administration of recombinant mouse leptin has not been carefully evaluated. Exogenous leptin was administered (3 mg leptin/kg body weight) to ten week old fasted non-obese male mice and plasma was serially collected at seven time points; plasma leptin concentration was measured by ELISA at each time point to estimate the circulating half life of mouse leptin. Under the physiological circumstances tested, the half life of mouse leptin was 40.2 (+/− 2.2) minutes. Circulating leptin concentrations up to one hour following exogenous leptin administration were 170-fold higher than endogenous levels at fasting. The half life of mouse leptin was determined to be 40.2 minutes. These results should be useful in planning and interpreting experiments employing exogenous leptin. The unphysiological elevations in circulating leptin resulting from widely used dosing regimens for exogenous leptin are likely to confound inferences regarding some aspects of the hormone’s clinical biology.