Apoptosis parallels ceramide content in the developing rat kidney.
Apoptosis parallels ceramide content in the developing rat kidney.
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细胞凋亡与发育中的大鼠肾脏中的神经酰胺含量相似。
DOI：
10.1007/s004670000444
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Chevalier,RL
中科院分区：
文献类型：
--
作者：
Malik,RK;Thornhill,BA;Chang,AY;Kiley,SC;Chevalier,RL
神经酰胺是一种重要的疏水性鞘磷脂，参与细胞分化和细胞凋亡。由于细胞凋亡在肾脏形态发生过程中的细胞重塑中起重要作用，我们测量了胎儿(妊娠18天)、新生儿(出生后3、7和14天)和成年大鼠肾脏的神经酰胺含量和细胞凋亡。此外，为了确定神经酰胺含量的发育变化是否具有组织特异性，我们将肾脏神经酰胺含量与肺和肝脏的神经酰胺含量进行了比较。神经酰胺的测定采用甘油二酯激动法，细胞凋亡的测定采用原位末端标记法。从胎儿到出生后7天，肾脏神经酰胺含量下降了100倍以上。肾脏细胞凋亡与神经酰胺含量平行，从胎儿到成年，细胞凋亡率减少了300多倍。肺和肝脏神经酰胺含量显著低于肾脏，且随成熟度变化较小。我们得出结论，神经酰胺含量的成熟变化是组织特异性的，发育中的肾脏高凋亡率可能与神经酰胺含量升高有关。
Ceramide is emerging as an important hydrophobic sphingolipid involved in cell differentiation and apoptosis. Since apoptosis plays a significant role in cellular remodeling during renal morphogenesis, we measured ceramide content and apoptosis in the fetal (18 days gestation), neonatal (3, 7, and 14 days postnatal), and adult rat kidney. In addition, to determine whether developmental changes in ceramide content are tissue-specific, we compared renal ceramide content with that in lung and liver. Ceramide was measured by the diacylglycerol kinase assay, and apoptosis was determined by the TUNEL technique. Renal ceramide content fell over 100-fold from the fetus to the 7th postnatal day. Renal apoptosis paralleled ceramide content, with a greater than 300-fold decrease in apoptosis from fetal to adult life. Ceramide content of the lung and liver was significantly less than that of the kidney, and changed less with maturation. We conclude that maturational changes in ceramide content are tissue-specific, and that the high rate of apoptosis in the developing kidney may be related to the elevated ceramide content.