FosL1 Regulates Regional Metastasis of Head and Neck Squamous Cell Carcinoma by Promoting Cell Migration, Invasion, and Proliferation
FosL1 Regulates Regional Metastasis of Head and Neck Squamous Cell Carcinoma by Promoting Cell Migration, Invasion, and Proliferation
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DOI：
10.21873/anticanres.15119
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发表时间：
2021-07-01
影响因子：
2
通讯作者：
Oridate, Nobuhiko
中科院分区：
文献类型：
--
作者：
Hyakusoku, Hiroshi;Sawakuma, Kae;Oridate, Nobuhiko
背景/目的：我们评估了活化蛋白-1家族成员FosL 1对导致头颈部鳞状细胞癌（HNSCC）区域转移的途径的影响。材料与方法：我们研究了小干扰RNA（siRNA）和短肝素RNA（shRNA）介导的FosL 1敲低对体外细胞迁移、侵袭和增殖以及体内区域转移的影响。还使用来自HNSCC患者数据库的数据使用Kaplan-Meier检验分析FosL 1的预后意义。结果如下：下调FosL 1的表达可抑制体外细胞迁移、侵袭和增殖，降低局部转移的发生率，并延长小鼠体内生存期。我们还确定了具有较高FosL 1表达水平的HNSCC患者的生存时间显著短于FosL 1低表达的患者。结论：FosL 1在促进HNSCC细胞迁移、侵袭和增殖中起重要作用。
Background/Aim: We evaluated the impact of FosL1, a member of the activated protein-1 family, on the pathways leading to regional metastasis of head and neck squamous cell carcinoma (HNSCC). Materials and Methods: We examined the influence of small interfering RNA (siRNA) and short heparin RNA (shRNA) mediated knockdown of FosL1 on cell migration, invasion, and proliferation in vitro as well as on regional metastasis in vivo. The prognostic significance of FosL1 was also analyzed using the Kaplan- Meier plotter using data from an HNSCC patient database. Results: Down-regulation of FosL1 inhibited cell migration, invasion, and proliferation in vitro, decreased the incidence of regional metastases, and prolonged the survival of mice in vivo. We also determined that HNSCC patients with higher expression levels of FosL1 had a significantly shorter survival time than those with low expression of FosL1. Conclusion: FosL1 plays a crucial role in promoting cell migration, invasion, and proliferation in HNSCC.