Monomolecular multimodal fluorescence-radioisotope imaging agents
Monomolecular multimodal fluorescence-radioisotope imaging agents
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DOI：
10.1021/bc050136s
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发表时间：
2005-09-01
影响因子：
4.7
通讯作者：
Achilefu, S
中科院分区：
文献类型：
--
作者：
Zhang, ZR;Liang, KX;Achilefu, S
通过不同的成像方法诊断疾病可以提供关于患病组织或器官的功能状态的补充信息。为了克服目前的困难，从不同的成像模式与高精度的图像配准，我们准备了近红外（NIR）单分子多模态成像剂（MOMIA）组成的七甲川羰花青和在-111-DOTA螯合物，作为天线的光学和荧光成像，分别。它们的光谱性质清楚地表明，铟与MOMIA的配位增加了化合物的荧光强度。MOMIA在生物培养基和血清中在37 ℃下长达24小时特别稳定。通过荧光光子和γ计数获得的化合物在小鼠中的生物分布表明，分子探针在不同组织中的分布趋势相似，表明检测到的荧光和γ发射来自同一来源（MOMIA）。在注射后24 h，MOMIA由肾脏和肝胆系统排泄，并且代表性MOMIA的血液水平非常低，从而降低了由循环分子探针引起的背景噪声。这些研究结果表明，制备具有发射可辨别和诊断荧光和伽马辐射的能力的单分子用于活体的光学和核成像的可行性。
Diagnosis of diseases by different imaging methods can provide complementary information about the functional status of diseased tissues or organs. To overcome the current difficulties in coregistering images from different imaging modalities with a high degree of accuracy, we prepared near-infrared (NIR) monomolecular multimodal imaging agents (MOMIAs) consisting of a heptamethine carbocyanine and In-111-DOTA chelate that served as antennae for optical and scintigraphic imaging, respectively. Their spectral properties clearly show that coordination of indium to MOMIA increased the fluorescence intensity of the compounds. The MOMIAs are exceptionally stable in biological media and serum up to 24 h at 37 degrees C. Biodistribution of the compounds in mice obtained by fluorescence photon and gamma-counts demonstrated a similar distribution trend of the molecular probe in different tissues, suggesting that the detected fluorescence and gamma-emissions emanated from the same source (MOMIA). At 24 h postinjection, the MOMIAs were excreted by the renal and hepatobiliary systems and the blood level of a representative MOMIA was very low, thereby reducing background noise caused by circulating molecular probes. These findings demonstrate the feasibility of preparing single molecules with the capacity to emit discernible and diagnostic fluorescent and gamma-radiations for optical and nuclear imaging of living organisms.