Quantum dot induced cellular perturbations involving varying toxicity pathways
Quantum dot induced cellular perturbations involving varying toxicity pathways
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DOI：
10.1039/c4tx00175c
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发表时间：
2015-04
影响因子：
2.1
通讯作者：
Abdullah Al-Ali;Neenu Singh;Bella B. Manshian;T. S. Wilkinson;J. Wills;G. Jenkins;S. Doak
中科院分区：
文献类型：
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作者：
Abdullah Al-Ali;Neenu Singh;Bella B. Manshian;T. S. Wilkinson;J. Wills;G. Jenkins;S. Doak
量子点（QD）独特的光学和电子特性使其开发和应用取得了快速进展，特别是在创新的治疗和诊断产品中。沿着量子点的快速发展，研究的重点是制备具有新颖表面功能的毒性较小的量子点。因此，本研究的重点是评估不同QD表面化学对细胞摄取的影响以及暴露后细胞扰动的一系列指标。该研究表明，尽管具有不同表面官能团的三种QD的内在细胞毒性较低，但它们都能够诱导急性炎症反应和转录基因活性的改变，而不影响细胞周期调控。此外，该研究表明，尽管QD能够诱导炎症和氧化应激反应，但根据表面化学，分子变化程度存在明显变化，这与细胞摄取程度相关。因此，这些发现突出了暴露于QD后慢性炎症反应的可能性，但此外，它们也证明了研究广泛的毒性途径以全面了解纳米材料生物反应的重要性。
The unique optical and electronic properties of quantum dots (QD) have led to rapid progress in their development and application, particularly in innovative therapeutic and diagnostic products. Along with the great pace at which QD are being developed, research is being focussed on fabricating less toxic QD with novel surface functionalities. The present study was therefore focused on assessing the impact of varying QD surface chemistry on cellular uptake and a range of indicators for cell perturbation following exposure. The study demonstrated that despite a low intrinsic cytotoxicity of three QD with different surface functional groups, they were all capable of inducing an acute inflammatory response and alterations in transcriptional gene activity, without affecting cell cycle regulation. Further, this investigation demonstrated that although the QD were capable of inducing an inflammatory and oxidative stress response, there was clearly variation in the degree of molecular change according to surface chemistry, which correlated with the degree of cellular uptake. These findings therefore highlight the potential for chronic inflammatory responses following exposure to QD, but in addition, they also demonstrate the importance of studying a wide range of toxicity pathways to generate a comprehensive picture of biological response to nanomaterials.