Comparative analysis of cherry virus A genome sequences assembled from deep sequencing data
Comparative analysis of cherry virus A genome sequences assembled from deep sequencing data
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DOI：
10.1007/s00705-017-3394-1
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发表时间：
2017-09-01
影响因子：
2.7
通讯作者：
Rott, Michael
中科院分区：
文献类型：
--
作者：
Kesanakurti, Prasad;Belton, Mark;Rott, Michael
樱桃病毒A（Cherry virus A，CVA）是一种普遍存在的嫁接传播病毒，主要侵染李属植物。下一代测序应用于39树果实标本感染CVA，75全长和16部分长度CVA基因组序列组装。对这些和11个先前测序的CVA基因组的系统发育分析产生了6个主要簇，在宿主和组装的基因组序列之间没有可观察到的关系。纯化分析检测到4个重组子。一致的单核苷酸多态性（SNP）模式之间观察到的75个全长基因组和它们的序列云，这支持CVA进化的准种模型。
Cherry virus A (CVA) is a ubiquitous graft-transmissible virus that mainly infects Prunus spp. Next-generation sequencing was applied to 39 tree fruit specimens infected with CVA, and 75 full and 16 partial-length CVA genome sequences were assembled. Phylogenetic analysis of these and 11 previously sequenced CVA genomes resulted in six major clusters with no observable relationship between the host and the assembled genome sequences. Recombination analysis detected four recombinants. Consistent single-nucleotide polymorphism (SNP) patterns were observed between the 75 full-length genomes and their sequence clouds, which supports a quasispecies model for CVA evolution.