Harvesting of Living Cell Sheets by the Dynamic Generation of Diffractive Photothermal Pattern on PEDOT
Harvesting of Living Cell Sheets by the Dynamic Generation of Diffractive Photothermal Pattern on PEDOT
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DOI：
10.1002/adfm.201604260
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发表时间：
2017-03
影响因子：
19
通讯作者：
Jongbeom Na;J. S. Heo;Minsu Han;Hanwhuy Lim;Hyun Ok Kim;Eunkyoung Kim
中科院分区：
文献类型：
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作者：
Jongbeom Na;J. S. Heo;Minsu Han;Hanwhuy Lim;Hyun Ok Kim;Eunkyoung Kim
导电聚合物薄膜上的近红外 (NIR) 光热图案使独特的方法能够在不使用图案培养皿的情况下收获具有各种图案的大面积细胞片。近红外光热图案由图案化光学透镜 (POL) 生成，可在聚合物薄膜上创建动态近红外光图案和相应的光热图案 (PTP)。 POL 由透明聚二甲基硅氧烷制成，旨在产生各种光图案。 PTP 允许以完整的活细胞片形式非侵入性地收获培养细胞，且收获效率高 (ηcell > 100)。通过近红外光通过具有不同微图案的 POL 的衍射产生各种 PTP，这些 PTP 提供具有所需图案的细胞片层，而不改变培养状态下的原始细胞形态。此外，还获得了大面积的活细胞片，其剥离面积大于19 cm2，这是迄今为止最大的活细胞片。采集系统的进一步光学工程允许用一剂光多次生产细胞片。不仅可以从人类成纤维细胞中收获细胞片，还可以从人类脂肪干细胞中收获细胞片，这表明该方法可以应用于改造各种细胞。
Near infrared (NIR) photothermal pattern on conductive polymer film enables unique approaches to harvest large‐area cell sheets with various patterns without the use of patterned culture dish. The NIR photothermal pattern is generated from a patterned optical lens (POL), which creates a dynamic near IR light pattern and the corresponding photothermal pattern (PTP) on the polymer film. The POL is prepared from transparent polydimethylsiloxane designed to generate various light patterns. The PTPs allow a noninvasive harvest of cultured cells as an intact living cell sheet with a high harvesting efficiency (ηcell > 100). Various PTPs are generated by the diffraction of NIR light through POLs having different micropatterns, which afford cell sheets with a desired pattern without changing the original cell morphology at cultured state. Furthermore, a large‐area living cell sheet is obtained with a detached area larger than 19 cm2, which is the largest living cell sheet up to date. Further optical engineering of the harvesting system allows multiple productions of cell sheets with one dose of light. It is possible to harvest cell sheets not only from human fibroblast cells but also from human adipose‐derived stem cells, indicating that the method can be applied to engineer various cells.