Structure of the complete, membrane-assembled COPII coat reveals a complex interaction network.
Structure of the complete, membrane-assembled COPII coat reveals a complex interaction network.
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DOI：
10.1038/s41467-021-22110-6
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发表时间：
2021-04-01
影响因子：
16.6
通讯作者：
Zanetti G
中科院分区：
文献类型：
--
作者：
Hutchings J;Stancheva VG;Brown NR;Cheung ACM;Miller EA;Zanetti G
COPII介导内质网到高尔基体的成千上万的货物运输。五种必需的蛋白质组装成两层结构，内层被认为是调节外套组装和货物招募，外层被认为是支架膜曲率。在这里，我们可视化完整的，膜组装COPII涂层的冷冻电子断层扫描和subtomography平均，揭示了完整的网络内和涂层之间的相互作用。我们使用遗传和生物化学方法证明了这些相互作用的生理重要性。突变表明，内涂层单独可以提供膜重塑功能，与组织输入从外涂层。这些功能作用的内，外涂层显着远离目前的范式，假定膜曲率主要来自外涂层。我们认为这些相互作用共同促进了外套组织和膜曲率，提供了一个结构框架，以了解COPII运输和分泌的调控机制。胞质外壳蛋白捕获分泌货物并塑造用于细胞内运输的膜载体，例如COPII，其介导内质网至高尔基体的数千货物运输。在这里，作者通过低温电子断层扫描和亚断层图像平均化来可视化完整的膜组装COPII涂层，揭示了涂层内和涂层之间相互作用的完整网络。
COPII mediates Endoplasmic Reticulum to Golgi trafficking of thousands of cargoes. Five essential proteins assemble into a two-layer architecture, with the inner layer thought to regulate coat assembly and cargo recruitment, and the outer coat forming cages assumed to scaffold membrane curvature. Here we visualise the complete, membrane-assembled COPII coat by cryo-electron tomography and subtomogram averaging, revealing the full network of interactions within and between coat layers. We demonstrate the physiological importance of these interactions using genetic and biochemical approaches. Mutagenesis reveals that the inner coat alone can provide membrane remodelling function, with organisational input from the outer coat. These functional roles for the inner and outer coats significantly move away from the current paradigm, which posits membrane curvature derives primarily from the outer coat. We suggest these interactions collectively contribute to coat organisation and membrane curvature, providing a structural framework to understand regulatory mechanisms of COPII trafficking and secretion. Cytosolic coat proteins capture secretory cargo and sculpt membrane carriers for intracellular transport, such as COPII which mediates Endoplasmic Reticulum to Golgi trafficking of thousands of cargoes. Here authors visualise the complete, membrane-assembled COPII coat by cryo-electron tomography and subtomogram averaging, revealing the full network of interactions within and between coat layers.
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影响因子：
16.6
作者：
Hutchings J;Stancheva V;Miller EA;Zanetti G
通讯作者：
Zanetti G
影响因子：
30.8
作者：
Boyadjiev, Simeon A.;Fromme, J. Christopher;Eyaid, Wafaa
通讯作者：
Eyaid, Wafaa
影响因子：
14.9
作者：
Buchan, Daniel W. A.;Jones, David T.
通讯作者：
Jones, David T.
DOI：
10.1083/jcb.201702135
发表时间：
2017-06-05
期刊：
The Journal of cell biology
影响因子：
--
作者：
Gorur A;Yuan L;Kenny SJ;Baba S;Xu K;Schekman R
通讯作者：
Schekman R
影响因子：
64.5
作者：
Lee, MCS;Orci, L;Schekman, R
通讯作者：
Schekman, R