Aquaporin7 plays a crucial role in tolerance to hyperosmotic stress and in the survival of oocytes during cryopreservation.
Aquaporin7 plays a crucial role in tolerance to hyperosmotic stress and in the survival of oocytes during cryopreservation.
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水通道蛋白7在高渗应激耐受性和冷冻保存期间卵母细胞的存活中发挥着至关重要的作用。
DOI：
10.1038/srep17741
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发表时间：
2015-12-04
影响因子：
4.6
通讯作者：
Huang HF
中科院分区：
文献类型：
--
作者：
Tan YJ;Zhang XY;Ding GL;Li R;Wang L;Jin L;Lin XH;Gao L;Sheng JZ;Huang HF
高渗应激可诱导不同细胞的凋亡。然而，卵母细胞在玻璃化冷冻保存过程中表现出对渗透胁迫的耐受性，玻璃化冷冻是一种辅助生殖技术。其潜在机制仍不清楚。在这里，我们证明了高浓度的冷冻保护剂，包括DMSO，乙二醇和蔗糖，产生的高渗透，显着上调水通道蛋白（AQP）7的蛋白水平，但不是AQP 3和AQP 9，在小鼠卵母细胞。通过siRNA注射敲低AQP 7表达显著降低了玻璃化冷冻后卵母细胞的存活率。在卵母细胞中，AQP 7与F-肌动蛋白结合，F-肌动蛋白是一种参与几乎所有生物事件的蛋白质。此外，我们发现高渗可以上调CPE结合蛋白（CPEB）和Aurora A的磷酸化水平。PI 3 K和PKC通路的抑制阻断了高血压诱导的AQP 7上调以及卵母细胞中CPEB和Aurora A的磷酸化。结论：高渗可通过PI 3 K和PKC介导的Aurora A/CPEB磷酸化上调AQP 7的表达，AQP 7的上调在提高卵母细胞玻璃化冷冻耐受性和存活率中起重要作用。
Hyperosmotic stress may induce apoptosis of different cells. However, oocytes show tolerance to osmotic stress during cryopreservation by vitrification, which is an assisted reproductive technique. The underlying mechanism is still not understood. Here, we demonstrated that hyperosmosis produced by high concentrations of cryoprotectants, including DMSO, ethylene glycol and sucrose, significantly upregulated the protein levels of aquaporin (AQP) 7, but not AQP3 and AQP9, in mouse oocytes. Knockdown of AQP7 expression by siRNA-injection significantly reduced the survival of oocytes after vitrification. In oocytes, AQP7 was shown to bind with F-actin, a protein involved in almost all biological events. Moreover, we found that hyperosmosis could upregulate the phosphorylation levels of CPE-binding protein (CPEB) and Aurora A. Inhibition of the PI3K and PKC pathways blocked the hyperosmosis-induced upregulation of AQP7 and the phosphorylation of CPEB and Aurora A in oocytes. In conclusion, hyperosmosis could upregulate the expression of AQP7 via Aurora A/CPEB phosphorylation mediated by the PI3K and PKC pathways, and upregulation of AQP7 plays an important role in improving of tolerance to hyperosmotic stress and survival of oocytes during cryopreservation by vitrification.