Performance of PCR-restriction fragment length polymorphism analysis of the Helicobacter pylori ureB gene in differentiating gene variants.
Performance of PCR-restriction fragment length polymorphism analysis of the Helicobacter pylori ureB gene in differentiating gene variants.
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幽门螺杆菌 ureB 基因 PCR 限制性片段长度多态性分析在区分基因变异中的表现。
DOI：
10.1046/j.1469-0691.2003.00476.x
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
L. P. Andersen
中科院分区：
文献类型：
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作者：
H. Colding;S. Hartzen;M. Mohammadi;K. Herup;J. Kristiansen;L. P. Andersen
最近，我们对幽门螺杆菌尿素酶基因的聚合酶链反应-限制性片段长度多态性（PCR-RFLP）进行了一项荟萃分析，发现尿素酶AB和C基因的可接受的区分指数。pylori分离株的ureB基因（933 bp）的PCR-RFLP分型，结合用限制性内切酶Hae III和Sau 3A以及这两种酶的混合物获得的结果。因此，我们发现H. pylori的限制性内切酶Hae Ⅲ和Sau 3A分型与其他H. pylori尿素酶基因。
Recently, PCR-restriction fragment length polymorphism (PCR-RFLP) of the urease genes of Helicobacter pylori was evaluated in a meta-analysis; acceptable discriminatory indices of the ureAB and C genes were found. In the present investigation, we found a discriminatory index of 0.95 for 191 unrelated clinical H. pylori isolates with PCR-RFLP typing of the ureB gene (933 bp), combining the results obtained with restriction enzymes HaeIII and Sau3A, and a mixture of the enzymes. We therefore find that PCR-RFLP typing of the ureB gene of H. pylori with restriction enzymes HaeIII and Sau3A is comparable to typing of other H. pylori urease genes.