In vitro synthesis of polyhydroxyalkanoate catalyzed by class II and III PHA synthases: a useful technique for surface coatings of a hydrophobic support with PHA
In vitro synthesis of polyhydroxyalkanoate catalyzed by class II and III PHA synthases: a useful technique for surface coatings of a hydrophobic support with PHA
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DOI：
10.1002/jctb.2261
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发表时间：
2009-09
影响因子：
3.4
通讯作者：
Shun Sato;M. Minato;Y. Kikkawa;H. Abe;Takeharu Tsuge
中科院分区：
文献类型：
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作者：
Shun Sato;M. Minato;Y. Kikkawa;H. Abe;Takeharu Tsuge
利用恶臭假单胞菌（Pseudomonasputida）的II类PHA合酶（PhaC 1 Pp）和葡萄酒异染色菌（Allochromatiumvinosum）的III类PHA合酶（PhaECAv）在疏水载体（即高取向热解石墨（HOPG））上进行聚羟基链烷酸酯（PHA）的体外合成。使用PhaC 1 Pp和3-羟基辛酰辅酶A，聚（3-羟基辛酸酯）[P（3 HO）]膜上形成的疏水性支持物的厚度为几个纳米，通过原子力显微镜（AFM）显示。还使用PhaECAv和3-羟基丁酰-CoA形成聚（3-羟基丁酸酯）[P（3 HB）]膜。AFM观察了P（3 HB）成膜过程中HOPG表面的形貌，发现在反应时间内聚合的P（3 HB）覆盖了HOPG表面，形成了均匀的超薄膜。这种在疏水载体上形成超薄PHA膜的方法可适用于具有生物可降解、生物可吸收和生物相容性PHA的材料的表面涂覆技术。版权所有© 2009化学工业协会
In vitro synthesis of polyhydroxyalkanoates (PHAs) on a hydrophobic support, i.e. highly oriented pyrolytic graphite (HOPG), was performed using class II PHA synthase (PhaC1Pp) from Pseudomonas putida and class III PHA synthase (PhaECAv) from Allochromatium vinosum. Using PhaC1Pp and 3-hydroxyoctanoyl-CoA, a poly(3-hydroxyoctanoate) [P(3HO)] film was formed on the hydrophobic support with a thickness of a few nanometers, as revealed by atomic force microscopy (AFM). A poly(3-hydroxybutyrate) [P(3HB)] film was also formed using PhaECAv and 3-hydroxybutyryl-CoA. AFM observations of the HOPG surface during P(3HB) film formation revealed that polymerized P(3HB) covered HOPG surface within the reaction time, resulting in the formation of a homogeneous ultra-thin film. This method of ultra-thin PHA film formation on a hydrophobic support may be applicable to the surface-coating technique of materials with biodegradable, bioabsorbable, and biocompatible PHAs. Copyright © 2009 Society of Chemical Industry