Menthol from Mentha Piperita Suppresses the Milk Production of Lactating Mammary Epithelial Cells In Vivo and In Vitro.
Menthol from Mentha Piperita Suppresses the Milk Production of Lactating Mammary Epithelial Cells In Vivo and In Vitro.
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DOI：
10.1002/mnfr.202000853
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发表时间：
2020-11
影响因子：
5.2
通讯作者：
Norihiro Suzuki;Yusaku Tsugami;Haruka Wakasa;Takahiro Suzuki;T. Nishimura;Ken Kobayashi
中科院分区：
文献类型：
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作者：
Norihiro Suzuki;Yusaku Tsugami;Haruka Wakasa;Takahiro Suzuki;T. Nishimura;Ken Kobayashi
范围薄荷传统上被用作母乳喂养妇女的抗催乳剂。然而，抑制机制仍不清楚。我们研究了薄荷是否以及如何在细胞和分子水平上影响产奶量。方法和结果我们制备了一个哺乳期乳腺上皮细胞（MEC）培养模型，产生主要的牛奶成分。薄荷油（PMO）抑制β-酪蛋白的产生与细胞质脂滴（CLD）的诱导扩大。PMO还显著灭活了催乳信号通路中的STAT 5和mTOR。作为主要PMO组分的甲孕酮治疗导致β-酪蛋白产生减少、CLD增大、STAT 5和mTOR失活。薄荷的其他成分桉叶醇和乙酸薄荷酯对哺乳期MEC没有抑制作用。薄荷醇给药后STAT 5和mTOR的失活在哺乳期小鼠的肺泡MEC中也很明显。此外，泌乳MEC表达TRPM 8和TRPA 1，它们是被称为冷受体的薄荷醇受体。TRPM 8和TRPA 1的激动剂抑制泌乳MEC中β-酪蛋白的产生以及STAT 5和mTOR的失活。结论薄荷具有潜在的催乳作用。薄荷脑被认为是薄荷中的活性化合物。TRPM 8和TRPA 1可能作为薄荷醇的受体发挥作用。本文受版权保护。All rights reserved.
SCOPE Peppermint is traditionally used as an antigalactagogue in breastfeeding women. However, the suppressive mechanism remains unclear. We investigated whether and how peppermint influences milk production at the cellular and molecular levels. METHODS AND RESULTS We prepared a lactating mammary epithelial cell (MEC) culture model that produced major milk components. Peppermint oil (PMO) suppressed β-casein production in conjunction with the induced enlargement of cytoplasmic lipid droplets (CLDs). PMO also significantly inactivated STAT5 and mTOR in the lactogenic signaling pathway. Menthol treatment, which is a primary PMO component, led to decreases in β-casein production, enlarged CLDs, the inactivated STAT5 and mTOR. Eucalyptol and menthyl acetate, other components of peppermint, did not show suppressive effects on lactating MECs. The inactivation of STAT5 and mTOR upon menthol administration was also evident in alveolar MECs of lactating mice. Furthermore, lactating MECs expressed TRPM8 and TRPA1, which are menthol receptors known as cold receptors. Agonists of TRPM8 and TRPA1 suppressed β-casein production and inactivation of STAT5 and mTOR in the lactating MECs. CONCLUSION These findings indicate that peppermint has potential as an antigalactagogue. Menthol is suggested to be an active compound in peppermint. TRPM8 and TRPA1 may function as receptors for menthol. This article is protected by copyright. All rights reserved.