Long-term egg-yolk adaptation of the Orientia tsutsugamushi for preparation of a formalinized immunogen
Long-term egg-yolk adaptation of the Orientia tsutsugamushi for preparation of a formalinized immunogen
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DOI：
10.1016/j.vaccine.2005.07.113
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发表时间：
2006-02-27
期刊：
影响因子：
5.5
通讯作者：
Ahn, BY
中科院分区：
文献类型：
--
作者：
Choi, Y;Kim, KS;Ahn, BY
为了评估制备针对恙虫病的灭活立克次体疫苗的可行性，通过在卵中连续传代超过100次超过9年的时间来适应恙虫病东方体的Karp和Gilliam株，以产生比未适应的立克次体多约5倍的抗原。用差速离心法和间歇式离子交换层析法纯化鸡卵生长的立克次体，并用福尔马林灭活。单株免疫原或两株免疫原联合免疫C3 H/He小鼠，均获得较强的保护性免疫，持续时间均超过8个月。然而，无论是免疫原保护动物的挑战与两个异源菌株或Boryong，在韩国流行的菌株，尽管这三个抗原蛋白的47，56，和110 kDa的两种制剂中是突出的。IgM，IgG，和中和抗体诱导免疫小鼠的水平和模式与活立克次体感染的动物。(c)2005爱思唯尔有限公司保留所有权利。
To assess the feasibility of preparing a killed rickettsial vaccine against the scrub typhus, the Karp and Gilliam strains of the Orientia tsutsugamushi were adapted through sequential passages in eggs for more than 100 times over 9 years to produce approximately five times more antigen than the unadapted rickettsia. The egg-grown rickettsia was purified by differential centrifugation and batch-type ion-exchange chromatography, and inactivated by formalin treatment. Strong protective immunity was acquired against lethal challenges of the homologous strains and lasted fully for longer than 8 months in the C3H/He mice immunized with either of the single-strain immunogen or in combination of the two strain immumogens. However, neither immunogen protected animals from the challenges with the two heterologous strains or Boryong, the prevalent strain in Korea, despite that three antigenic proteins of 47, 56, and 110 kDa were eminent in both preparations. IgM, IgG, and neutralizing antibody were induced in the immunized mice in a level and pattern comparable to that in animals infected with live rickettsiae. (c) 2005 Elsevier Ltd. All rights reserved.