Biological phosphorus removal by pure culture of Lampropedia spp.
Biological phosphorus removal by pure culture of Lampropedia spp.
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DOI：
10.1016/s0043-1354(96)00351-x
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发表时间：
1997-06
期刊：
影响因子：
12.8
通讯作者：
L. Stante;C. M. Cellamare;F. Malaspina;G. Bortone;A. Tilche
中科院分区：
文献类型：
--
作者：
L. Stante;C. M. Cellamare;F. Malaspina;G. Bortone;A. Tilche
光足虫属是一种革兰氏阴性、奈瑟氏阳性球菌，其分离自在奶牛场和养猪场废水上操作的EBPR（增强生物除磷）活性污泥实验室工厂。在对乙酸钠进行的需氧生长试验中，丽足藻属（Lampropedia spp.）储存的PHB高达12%w/w。生物量产量估计为0.55 g VSS.g−1HAc，比生长速率为0.045 h−1。实验最大乙酸去除率为71.86 mg HAc.g− 1VSS. h − 1，半饱和常数为71.78 mg.l−1。进行批次测试以检查是否存在丽足虫属。能够强化生物除磷。在厌氧条件下，Lampropedia spp.螯合的乙酸盐和储存的PHB，平均转换因子为0.33 mg PHB.mg-1HAc。测得的最大PHB储存容量为31%w/w，最大比PHB积累速率为17 mg PHB.g− 1VSS. h − 1，比厌氧乙酸盐吸收速率为57 mg HAc.g− 1VSS. h −1。磷的释放和乙酸盐的吸收之间的实验比率很低，平均为0.044 mg PO 4-P. mg-1HAc，在pH 7.5时，比速率范围为1.7至3.6 mg PO 4-P.g-1VSS. h-1。尽管这个数字很低，但分馏分析表明，在厌氧条件下，释放的磷酸盐来自细胞多磷酸盐降解。因此，所有的结果允许我们得出结论，Lampropedia spp.可以被分类为聚磷细菌
Lampropedia spp. is a Gram-negative, Neisser-positive coccus that was isolated from EBPR (enhanced biological phosphate removal) activated sludge laboratory plants operating on dairy and piggery wastewaters. In aerobic growth tests carried out on sodium acetate, Lampropedia spp. stored PHB up to 12% w/w. Biomass yield was estimated at 0.55 g VSS.g−1HAc and specific growth rate at 0.045 h−1. The experimental maximum acetic acid removal rate was 71.86 mg HAc.g−1VSS.h−1with a semisaturation constant of 71.78 mg.l−1. Batch tests were carried out to check whether Lampropedia spp. was capable of enhanced biological phosphorus removal. Under anaerobic conditions, Lampropedia spp. sequestered acetate and stored PHB with an average conversion factor of 0.33 mg PHB.mg−1HAc. The measured maximum PHB storage capacity was 31% w/w, with a maximum specific PHB accumulation rate of 17 mg PHB.g−1VSS.h−1and a specific anaerobic acetate uptake rate of 57 mg HAc.g−1VSS.h−1. The experimental ratio between phosphorus released and acetate taken up was low, on average 0.044 mg PO4-P.mg−1HAc, with a specific rate ranging from 1.7 to 3.6 mg PO4-P.g−1VSS.h−1at pH 7.5. Despite the low figure, fractionation analyses showed that in anaerobic conditions the released phosphate comes from cell polyphosphate degradation. Therefore, all the results allow us to conclude that Lampropedia spp. can be classified amongst the phosphorus accumulating bacteria