Comparative genomics reveals high biological diversity and specific adaptations in the industrially and medically important fungal genus Aspergillus.
Comparative genomics reveals high biological diversity and specific adaptations in the industrially and medically important fungal genus Aspergillus.
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比较基因组学揭示了工业和医学上重要的曲霉属真菌的高度生物多样性和特异性适应性
DOI：
10.1186/s13059-017-1151-0
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发表时间：
2017-02-14
期刊：
影响因子：
12.3
通讯作者：
Grigoriev IV
中科院分区：
文献类型：
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作者：
de Vries RP;Riley R;Wiebenga A;Aguilar-Osorio G;Amillis S;Uchima CA;Anderluh G;Asadollahi M;Askin M;Barry K;Battaglia E;Bayram Ö;Benocci T;Braus-Stromeyer SA;Caldana C;Cánovas D;Cerqueira GC;Chen F;Chen W;Choi C;Clum A;Dos Santos RA;Damásio AR;Diallinas G;Emri T;Fekete E;Flipphi M;Freyberg S;Gallo A;Gournas C;Habgood R;Hainaut M;Harispe ML;Henrissat B;Hildén KS;Hope R;Hossain A;Karabika E;Karaffa L;Karányi Z;Kraševec N;Kuo A;Kusch H;LaButti K;Lagendijk EL;Lapidus A;Levasseur A;Lindquist E;Lipzen A;Logrieco AF;MacCabe A;Mäkelä MR;Malavazi I;Melin P;Meyer V;Mielnichuk N;Miskei M;Molnár ÁP;Mulé G;Ngan CY;Orejas M;Orosz E;Ouedraogo JP;Overkamp KM;Park HS;Perrone G;Piumi F;Punt PJ;Ram AF;Ramón A;Rauscher S;Record E;Riaño-Pachón DM;Robert V;Röhrig J;Ruller R;Salamov A;Salih NS;Samson RA;Sándor E;Sanguinetti M;Schütze T;Sepčić K;Shelest E;Sherlock G;Sophianopoulou V;Squina FM;Sun H;Susca A;Todd RB;Tsang A;Unkles SE;van de Wiele N;van Rossen-Uffink D;Oliveira JV;Vesth TC;Visser J;Yu JH;Zhou M;Andersen MR;Archer DB;Baker SE;Benoit I;Brakhage AA;Braus GH;Fischer R;Frisvad JC;Goldman GH;Houbraken J;Oakley B;Pócsi I;Scazzocchio C;Seiboth B;vanKuyk PA;Wortman J;Dyer PS;Grigoriev IV
背景：曲霉属真菌对人类至关重要。物种包括具有工业应用的物种、人类、动物和农作物的重要病原体、食品中强致癌污染物的来源以及重要的遗传模型。八曲霉菌的基因组序列已经探讨了真菌生物学方面的调查，提出了进化和专业化的问题，在此genus.Results：我们已经产生了10个新的，高度多样化的曲霉属物种的基因组序列，并详细比较了这些姐妹和更遥远的属。真菌生物学的关键方面，包括初级和次级代谢，胁迫反应，生物量降解和信号转导的比较研究，揭示了物种之间的保护和多样性。观察到的基因组差异与实验研究进行了验证。这揭示了几个亮点，如无性物种的性别潜力，有机酸生产基因是黑曲霉的一个关键特征，降解植物生物量的替代方法，以及胁迫反应的遗传基础的迹象。全基因组系统发育分析详细展示了新的基因组测序的物种与其他aspergilli.Conclusions的关系：真菌物种之间的生物学差异的许多方面不能解释从基因组序列获得的现有知识。比较基因组学和实验研究，在这里，允许第一次全属的观点曲霉菌的生物多样性，并在许多情况下，但不是所有的情况下，基因组差异表型。所获得的见解可用于真菌的生物技术和医学应用。
Background:The fungal genus Aspergillus is of critical importance to humankind. Species include those with industrial applications, important pathogens of humans, animals and crops, a source of potent carcinogenic contaminants of food, and an important genetic model. The genome sequences of eight aspergilli have already been explored to investigate aspects of fungal biology, raising questions about evolution and specialization within this genus.Results:We have generated genome sequences for ten novel, highly diverse Aspergillus species and compared these in detail to sister and more distant genera. Comparative studies of key aspects of fungal biology, including primary and secondary metabolism, stress response, biomass degradation, and signal transduction, revealed both conservation and diversity among the species. Observed genomic differences were validated with experimental studies. This revealed several highlights, such as the potential for sex in asexual species, organic acid production genes being a key feature of black aspergilli, alternative approaches for degrading plant biomass, and indications for the genetic basis of stress response. A genome-wide phylogenetic analysis demonstrated in detail the relationship of the newly genome sequenced species with other aspergilli.Conclusions:Many aspects of biological differences between fungal species cannot be explained by current knowledge obtained from genome sequences. The comparative genomics and experimental study, presented here, allows for the first time a genus-wide view of the biological diversity of the aspergilli and in many, but not all, cases linked genome differences to phenotype. Insights gained could be exploited for biotechnological and medical applications of fungi.