Comparative analysis of layer-specific genes in mammalian neocortex
Comparative analysis of layer-specific genes in mammalian neocortex
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DOI：
10.1093/cercor/bhl102
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发表时间：
2007-08-01
期刊：
影响因子：
3.7
通讯作者：
Yamamori, Tetsuo
中科院分区：
文献类型：
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作者：
Watakabe, Akiya;Ichinohe, Noritaka;Yamamori, Tetsuo
我们用荧光双原位杂交技术检测了4个层特异性基因在猴和小鼠大脑皮质中的表达模式。根据它们的共表达谱，我们能够区分出深层神经元的几个亚群。一组以Er81的表达和Nurr1mRNAs的缺失为特征，主要定位于第5层。在猴子中，这个神经元组又被5-HT2C受体的mRNA表达进一步细分。5-HT2C(+)/Er81(+)神经元在大部分皮质区域位于5B层，但在初级视区(V1)分布于第6层。猴6层的另一组神经元以Nurr1mRNA表达为特征，在6A层和6B层分别细分为Nurr1(+)/结缔组织生长因子(CTGF)(-)和Nurr1(+)/CTGF(+)神经元。在猴中，Nurr1(+)/CTGF(+)神经元共表达Er81mRNA，而在小鼠中不表达。在3只猕猴注射示踪剂的基础上，我们发现6A层的Nurr1(+)神经元发出一些皮质投射，而不是皮质白质投射。虽然Nurr1(+)/CTGF(-)神经元仅限于小鼠皮质的外侧区域，但它们存在于整个猴子的皮质。因此，对于具有不同基因表达谱的神经元亚群，揭示了跨区域和跨物种的架构异质性V。
We examined the expression patterns of 4 layer-specific genes in monkey and mouse cortices by fluorescence double in situ hybridization. Based on their coexpression profiles, we were able to distinguish several subpopulations of deep layer neurons. One group was characterized by the expression of ER81 and the lack of Nurr1 mRNAs and mainly localized to layer 5. In monkeys, this neuronal group was further subdivided by 5-HT2C receptor mRNA expression. The 5-HT2C(+)/ER81(+) neurons were located in layer 5B in most cortical areas, but they intruded layer 6 in the primary visual area (V1). Another group of neurons, in monkey layer 6, was characterized by Nurr1 mRNA expression and was further subdivided as Nurr1(+)/connective tissue growth factor (CTGF)(-) and Nurr1(+)/CTGF(+) neurons in layers 6A and 6B, respectively. The Nurr1(+)/CTGF(+) neurons coexpressed ER81 mRNA in monkeys but not in mice. On the basis of tracer injections in 3 monkeys, we found that the Nurr1(+) neurons in layer 6A send some corticocortical, but not corticopulvinar, projections. Although the Nurr1(+)/CTGF(-) neurons were restricted to lateral regions in the mouse cortex, they were present throughout the monkey cortex. Thus, an architectonic heterogeneitV across areas and species was revealed for the neuronal subpopulations with distinct gene expression profiles.