The ichthyosis mouse: histologic, histochemical, ultrastructural, and autoradiographic studies of interfollicular epidermis.
The ichthyosis mouse: histologic, histochemical, ultrastructural, and autoradiographic studies of interfollicular epidermis.
复制标题
鱼鳞病小鼠：滤泡间表皮的组织学、组织化学、超微结构和放射自显影研究。
DOI：
10.1111/1523-1747.ep12485145
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发表时间：
1977
期刊：
影响因子：
--
通讯作者：
N. Esterly
中科院分区：
文献类型：
--
作者：
J. E. Jensen;N. Esterly
用组织学、组织化学、超微结构和放射自显影技术研究了隐性突变型小鼠鱼鳞病（ic/ic）背部和尾部的滤泡间表皮，并与杂合子和瑞士S小鼠表皮进行了比较。在ic/ic小鼠中，角质层增厚，颗粒层突出，棘层增生。某些呼吸酶和溶酶体酶的染色反应在背部和尾部的表皮中更明显。IC/IC表皮的超微结构研究表明过度丛生张力细丝和线粒体，核糖核蛋白颗粒，并在棘层细胞的膜涂层颗粒的数量增加。棘层和颗粒层细胞之间的细胞间连接处扩张，充满了膜涂层和无定形物质。颗粒层细胞中可见丰富的角蛋白形成结构、异常大的透明角质颗粒和持久的线粒体。在角质层中，内含物突出并持续进入细胞的上层，其轮廓不规则且大大增厚。三种小鼠的表皮细胞通过时间或标记指数无差异。
Interfollicular epidermis from back and tail of the recessive mutant mouse ichthyosis (ic/ic) was studied by histologic, histochemical, ultrastructural, and autoradiographic techniques and compared to heterozygous and Swiss S mouse epidermis. In the ic/ic mouse the stratum corneum was thickened, the granular layer prominent, and the stratum spinosum hyperplastic. Staining reactions for certain respiratory and lysosomal enzymes were more pronounced in epidermis of both back and tail. Ultrastructural studies of ic/ic epidermis demonstrated excessively clumped tonofilaments and increased numbers of mitochondria, ribonuclear protein particles, and membrane-coating granules in the stratum spinosum cells. Dilated intercellular junctions between the stratum spinosum and stratum granulosum cells were packed with membrane-coating and amorphous material. Profuse keratin-forming structures, abnormally large keratohyaline granules, and persistent mitochondria were seen in the stratum granulosum cells. In the stratum corneum, inclusions were prominent and persisted into the upper layers of cells, which were irregular in outline and greatly thickened. No differences in epidermal cell transit time or labeling index were demonstrated among the three types of mice.