Detection of CD4+ autoreactive T cells in T1D using HLA class II tetramers
Detection of CD4+ autoreactive T cells in T1D using HLA class II tetramers
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DOI：
10.1196/annals.1288.009
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发表时间：
2003-01-01
期刊：
影响因子：
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通讯作者：
Nepom, GT
中科院分区：
文献类型：
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作者：
Reijonen, H;Kwok, WW;Nepom, GT
可溶性11类MHC四聚体的使用使得能够在1型糖尿病患者的外周血中鉴定自身抗原特异性T细胞。我们的方法利用了由含有GAD 65肽的固定的11类MHC单体诱导的表达CD 25（+）/CD 4（高+）表型的高度活化的T细胞的出现。几乎所有的T细胞，染色的特定四聚体居住在这个群体中，因为这种激活模式是不存在于正常受试者，它可以提供一个有用的工具，用于分析自身免疫性糖尿病的T细胞反应。利用四聚体技术检测自身反应性T细胞是深入了解自身免疫分子基础机制的有力工具。T细胞的表型应提供用于免疫介导的β细胞反应性进展的有用标志物，并且可用于临床试验以评估靶向干预/预防自身免疫性疾病的免疫调节疗法的功效。
The use of soluble class 11 MHC tetramers has enabled the identification of autoantigen-specific T cells in the peripheral blood of type 1 diabetes patients. Our approach takes advantage of the appearance of highly activated T cells expressing a CD25(+)/CD4(high+) phenotype induced by immobilized class 11 MHC monomer containing the GAD65 peptide. Almost all T cells that stain with the specific tetramer reside in this population and, since this activation profile is not present in normal subjects, it may provide a useful tool for analysis of the T cell response in autoimmune diabetes. The utilization of tetramer techniques in the detection of autoreactive T cells is a powerful tool to gain insight into mechanisms of the molecular basis of autoimmunity. The phenotyping of T cells should provide useful markers for progression of immune-mediated beta cell reactivity and can be utilized in clinical trials to evaluate the efficacy of the immunomodulatory therapies targeting intervention/prevention of autoimmune diseases.