Biochemical and Cellular Assays to Study Mechanisms of PCP Signaling in Axon Guidance.
Biochemical and Cellular Assays to Study Mechanisms of PCP Signaling in Axon Guidance.
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用于研究轴突引导中 PCP 信号传导机制的生化和细胞测定。
DOI：
10.1007/978-1-0716-2035-9_19
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Zou,Yimin
中科院分区：
文献类型：
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作者：
Marfull-Oromí,Pau;Miller,KathrynM;Zou,Yimin
了解PCP成分如何调节轴突引导的生化和细胞机制对于了解大脑发育非常重要，并可能导致神经修复的新治疗方法。同时，轴突生长锥是一种高度极化的结构，是一个很好的实验系统。因此，这些新的机制，我们发现的轴突指导可能适用于PCP信号一般。在本章中，我们将介绍我们使用或开发的一些技术：（1）蛋白质定位和运输;（2）蛋白质磷酸化;（3）同一细胞中和两个相邻细胞中的蛋白质-蛋白质相互作用。
Understanding biochemical and cellular mechanisms of how PCP components regulate axon guidance is important for understanding brain development and may lead to new therapeutic approaches for neural repair. Meanwhile, axonal growth cones are a highly polarized structure and are a great experimental system. Therefore, some of these novel mechanisms we are uncovering for axon guidance may be applicable for PCP signaling in general. In this chapter, we introduce some of the techniques we used or developed: (1) protein localization and trafficking; (2) protein phosphorylation; and (3) protein–protein interactions in the same cell and across the two neighboring cells.