Effects of anti-intercellular adhesion molecule-1 antibody on reperfusion injury induced by late reperfusion in the rat middle cerebral artery occlusion model
Effects of anti-intercellular adhesion molecule-1 antibody on reperfusion injury induced by late reperfusion in the rat middle cerebral artery occlusion model
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DOI：
10.1097/00006123-200210000-00033
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发表时间：
2002-10-01
期刊：
影响因子：
4.8
通讯作者：
Sakaki, T
中科院分区：
文献类型：
--
作者：
Kanemoto, Y;Nakase, H;Sakaki, T
目的：炎症过程与再灌注、损伤机制有关。再灌注时白细胞迁移到缺血组织中，涉及与内皮细胞的细胞间粘附分子（ICAM）结合，被认为会加剧组织损伤。本研究的目的是评估抗 ICAM-1 抗体对大鼠大脑中动脉闭塞 (MCAO) 缝合模型后期再灌注后再灌注引起的损伤的影响。 方法：将动物分为四组：1) I 组 (n = 7)，6 小时永久性 MCAO； 2.) 第 2 组 (n = 7)，3 小时 MCAO，然后再灌注 3 小时 3) 第 3 组 (n = 6)，6 小时永久性 MCAO，并在 MCAO 发作后 2 小时用抗 ICAM-1 抗体（命名为 1A29，1 mg/kg）治疗； 4) 第 4 组（n = 6），3 小时 MCAO，随后 3 小时再灌注和 1A29 治疗。在实验过程中，通过激光多普勒血流扫描技术测量局部脑血流量。 6 h时间点处死所有大鼠，检测髓过氧化物酶活性白细胞浸润结果和2,3,5-三苯基氯化四氮唑染色组织学分析结果。 结果：MCAO前后局部脑血流值四组间无显着差异。再灌注后两个再灌注组的局部脑血流量值没有显着差异。第 2 组（再灌注组）的总脑区和皮质区的脑损伤体积百分比以及活动中的髓过氧化物酶年龄大脑均显着大于其他组（P < 0.05），但抗 ICAM-1 抗体治疗组 4 降低，P < 0.05）。然而，无再灌注的第2组与第1组和第3组之间没有差异。髓过氧化物酶活性与梗塞体积呈正相关（P < 0.01）。结论：本研究的结果表明，抗ICAM-1抗体治疗可有效抑制晚期动脉再通引起的早期炎症过程和再灌注诱导的损伤，这将有助于扩大溶栓治疗的治疗窗。
OBJECTIVE: Inflammatory processes have been implicated in the mechanisms of reperfusion, injury. The migration of leukocytes into ischemic tissue on reperfusion, which involves binding to the intercellular,adhesion molecule (ICAM) of the endothelial cell, is thought to exacerbate tissue injury. The aim of the present study was to assess the effects of an anti-ICAM-1 antibody on reperfusion-induced injury after late reperfusion in a rat middle cerebral artery occlusion (MCAO) suture model.METHODS: The animals were divided into four groups: 1) Group I (n = 7), 6 hours of permanent MCAO; 2.) Group 2 (n = 7), 3 hours of MCAO followed by 3 hours of reperfusion 3) Group 3 (n = 6), 6 hours of permanent,MCAO and treatment with anti-ICAM-1 antibody (designated 1A29, 1 mg/kg) at 2 hours after onset of MCAO; and 4) Group 4 (n = 6), 3 hours of MCAO followed by 3 hours of reperfusion and 1A29 treatment. During the experiment, regional cerebral blood flow was measured by a laser Doppler flowmetric scanning technique. At the 6-hour time point, all rats were killed,, and the results of leukocyte infiltration by myeloperoxidase activity and histological analysis using 2,3,5-triphenyltetrazolium chloride staining were examined.RESULTS: Regional cerebral blood flow values before and after MCAO were not significantly different among the four groups. Regional cerebral blood flow values after reperfusion were not Significantly different, in the two reperfused groups. The percentage brain injury volumes in both the total and cortical areas and the myeloperoxidase age brain in activity in the latter were significantly, larger in Group 2 (the reperfused group) than in the other groups (P < 0.05) but were decreased by anti-ICAM-1 antibody treatment Group 4, P < 0.05). However, there were no differences between (Group 2 versus. Groups 1 and 3 without reperfusion. Myeloperoxidase, activities correlated positively with infarct volumes (P < 0.01).CONCLUSION: The findings of this study demonstrate that the anti-ICAM-1 antibody treatment is effective at inhibiting early. inflammatory processes and reperfusion-induced injury caused by late arterial recanalization, which would contribute to widening the therapeutic window of thrombolytic,therapy.