Integration of human mesenchymal stem cells into the Wolffian duct in chicken embryos
Integration of human mesenchymal stem cells into the Wolffian duct in chicken embryos
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DOI：
10.1016/j.bbrc.2009.05.052
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发表时间：
2009-07-31
影响因子：
3.1
通讯作者：
Okabe, Masataka
中科院分区：
文献类型：
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作者：
Fukui, Akira;Yokoo, Takashi;Okabe, Masataka
发育中的动物胚胎为人类间充质干细胞（hMSCs）提供了物种间分化的适宜环境。我们之前已经证明，移植到大鼠胚胎后肾间质区域的hMSCs分化为肾特异性细胞。在这里，我们评估了hMSCs移植到鸡胚输尿管芽祖区时是否能够分化为收集管系统的前体，因为鸡胚比哺乳动物胚胎更容易操作和培养。将表达pax2的hMSCs移植到鸡输尿管芽祖区后，随着Wolffian管的延长向尾端迁移，并整合到Wolffian管上皮中。此外，鸡表达pax2的hMSCs在整合到沃尔夫管上皮后开始表达人的LIM1。这些结果表明，鸡表达pax2的hMSCs可以在鸡局部信号的影响下向沃尔夫管细胞分化。(C) 2009爱思唯尔公司版权所有。
Developing animal embryos have been providing human mesenchymal stern cells (hMSCs) with an appropriate environment for their differentiation between species. We previously demonstrated that hMSCs transplanted into the metanephric mesenchyme region of rat embryos differentiate into kidney-specific cells. Here, we assessed whether hMSCs are competent to differentiate into precursors of the collecting duct system when they are transplanted into the ureteric bud progenitor region of chicken embryos that are easier to be manipulated and cultured than mammalian embryos. When chicken Pax2-expressing hMSCs were transplanted into the chicken ureteric bud progenitor region, they migrated caudally with the elongating Wolffian duct and then were integrated into the Wolffian duct epithelia. Also, chicken Pax2-expressing hMSCs started to express human LIM1 after their integration into the Wolffian duct epithelia. These results suggest that chicken Pax2-expressing hMSCs can be competent to differentiate into the Wolffian duct cells by the influence of chicken local signals. (C) 2009 Elsevier Inc. All rights reserved.