A Metaproteomic Analysis of the Response of a Freshwater Microbial Community under Nutrient Enrichment.
A Metaproteomic Analysis of the Response of a Freshwater Microbial Community under Nutrient Enrichment.
复制标题
DOI：
10.3389/fmicb.2016.01172
复制
发表时间：
2016
影响因子：
5.2
通讯作者：
Pandhal J
中科院分区：
文献类型：
--
作者：
Russo DA;Couto N;Beckerman AP;Pandhal J
富营养化可导致藻类生物量失控增加，从而对整个微生物和远洋生物群落产生影响。研究表明，营养丰富如何影响微生物物种演替，但有关其对群落功能的影响的细节很少。在这里，我们应用代谢蛋白质组学的方法来研究在淡水微宇宙中，随着时间的推移，在贫营养和富营养条件下，藻类和细菌群落的功能变化。样本是在藻类和蓝藻占优势的早期采集的，后来在细菌占优势的情况下采集。来自两个处理和两个时间点的1048个蛋白质被鉴定，并用它们的指数修正的蛋白质丰度指数进行定量。在贫营养条件下，类杆菌表达胞外水解酶和Ton-B依赖受体来降解和运输附着在藻圈上的高分子化合物。α-和β-蛋白细菌从藻类渗出物(分别是碳水化合物和氨基酸)中捕获不同的底物，这表明资源分配以避免直接竞争。在富营养条件下，蓝藻的环境适应蛋白表明，与低碳营养丰富的环境中的藻类相比，蓝藻具有更好的韧性。这项研究深入了解了不同时间点低营养和富营养条件下功能微生物过程的差异，并强调了初级生产者如何控制淡水环境中的细菌资源。数据已存放到标识符为PXD004592的ProteomeXchange。
Eutrophication can lead to an uncontrollable increase in algal biomass, which has repercussions for the entire microbial and pelagic community. Studies have shown how nutrient enrichment affects microbial species succession, however details regarding the impact on community functionality are rare. Here, we applied a metaproteomic approach to investigate the functional changes to algal and bacterial communities, over time, in oligotrophic and eutrophic conditions, in freshwater microcosms. Samples were taken early during algal and cyanobacterial dominance and later under bacterial dominance. 1048 proteins, from the two treatments and two timepoints, were identified and quantified by their exponentially modified protein abundance index. In oligotrophic conditions, Bacteroidetes express extracellular hydrolases and Ton-B dependent receptors to degrade and transport high molecular weight compounds captured while attached to the phycosphere. Alpha- and Beta-proteobacteria were found to capture different substrates from algal exudate (carbohydrates and amino acids, respectively) suggesting resource partitioning to avoid direct competition. In eutrophic conditions, environmental adaptation proteins from cyanobacteria suggested better resilience compared to algae in a low carbon nutrient enriched environment. This study provides insight into differences in functional microbial processes between oligo- and eutrophic conditions at different timepoints and highlights how primary producers control bacterial resources in freshwater environments. The data have been deposited to the ProteomeXchange with identifier PXD004592.