Coexpression of ang1 and tie2 in odontoblasts of mouse developing and mature teeth-a new insight into dentinogenesis.
Coexpression of ang1 and tie2 in odontoblasts of mouse developing and mature teeth-a new insight into dentinogenesis.
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DOI：
10.1267/ahc.13043
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发表时间：
2014-02-27
影响因子：
2.4
通讯作者：
Ikeda T
中科院分区：
文献类型：
--
作者：
Nakajima K;Shibata Y;Hishikawa Y;Suematsu T;Mori M;Fukuhara S;Koji T;Sawase T;Ikeda T
血管生成素-1调节血管生成和稳定，并据报道通过促进血管生成来促进骨形成。为了探讨Ang 1在牙发生中的作用，我们研究了Ang 1及其受体Tie 2在小鼠下颌第一磨牙发育和成熟过程中的分布。在胚胎第18天，当成牙本质细胞分化开始时，Ang 1的免疫信号在基底膜和远端侧，面向成牙本质细胞的基底膜中被强烈检测到。原位杂交结果显示Ang 1在成牙本质细胞和成釉细胞中表达，且表达部位与基底膜相对。Tie 2定位于成牙本质细胞的远中端。出生后，Ang 1在前牙本质中被检测到，而Ang 1和Tie 2在成牙本质细胞和成牙本质细胞过程中共定位。这些分布保留长达8周。与成牙本质细胞相反，成釉细胞、成牙骨质细胞和成骨细胞表达Ang 1，但不表达Tie 2。Ang 1和Tie 2在成牙本质细胞中的共定位以及Tie 2在成牙本质细胞中的选择性表达表明Ang 1-Tie 2的自分泌信号参与牙本质形成。
Angiopoietin-1 regulates vascular angiogenesis and stabilization, and is reported to promote bone formation by facilitating angiogenesis. To estimate the role of Ang1 in odontogenesis, we explored the distribution of Ang1 and the receptor, Tie2 in the mouse developing and mature first molar of the mandible. At embryonic day 18, when differentiation of odontoblasts begins, immunosignals for Ang1 were intensely detected in the basement membrane and the distal side, which faced the basement membrane of odontoblasts. In situ hybridization revealed that Ang1 was expressed in odontoblasts and ameloblasts facing the basement membrane. Tie2 was localized in the distal side of odontoblasts. After birth, Ang1 was detected in the predentin, whereas both Ang1 and Tie2 were colocalized in odontoblasts and odontoblast processes. These distributions were retained up to 8 weeks. In contrast to odontoblasts, ameloblasts, cementoblasts and osteoblasts expressed Ang1 but did not express Tie2. Colocalization of Ang1 and Tie2 in odontoblasts and selective expression of Tie2 in odontoblasts among cells responsible for calcified tissue formation suggested the involvement of autocrine signals of Ang1-Tie2 in dentinogenesis.