Alterations in gut bacterial and fungal microbiomes are associated with bacterial Keratitis, an inflammatory disease of the human eye
Alterations in gut bacterial and fungal microbiomes are associated with bacterial Keratitis, an inflammatory disease of the human eye
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DOI：
10.1007/s12038-018-9798-6
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发表时间：
2018-12-01
影响因子：
2.9
通讯作者：
Shivaji, Sisinthy
中科院分区：
文献类型：
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作者：
Jayasudha, Rajagopalaboopathi;Chakravarthy, Sama Kalyana;Shivaji, Sisinthy
肠道菌群失调或失衡与自身免疫、炎症、神经系统疾病以及癌症有关。最近，它也被证明与眼部疾病有关。在本研究中，研究了肠道微生物群失调与细菌性角膜炎（一种可导致角膜失明的炎症性眼病）之间的关系。使用健康对照（HC，n = 21）和细菌性角膜炎患者（BK，n = 19）的粪便样本分析细菌和真菌肠道微生物组。与 BK 患者相比，HC 患者的细菌微生物组中多种抗炎微生物的丰度有所增加，包括 Dialister、Megasphaera、Faecalibacter、Lachnospira、瘤胃球菌和 Mitsuokella 以及厚壁菌门、Veillonellaceae、Ruminococcaceae 和 Lachnospiraceae 的成员。在真菌微生物组中，与 HC 相比，BK 患者中被孢霉属、根霉属、克鲁维酵母属、Embellisia 和 Haematonectria 的丰度减少，而病原真菌曲霉属和马拉色菌的丰度增加。此外，热图、PCoA 图和推断的功能图谱也表明 HC 和 BK 微生物组之间存在显着差异，这强烈表明 BK 患者肠道微生物组存在菌群失调。这是第一项证明肠道微生物组与 BK 病理生理学关联的研究，从而支持肠-眼轴假说。考虑到角膜炎每年影响全球约 100 万人，这些数据可能成为制定替代治疗策略的基础，例如使用益生菌或粪便移植来恢复健康的微生物群，作为角膜炎的治疗方案。
Dysbiosis, or imbalance in the gut microbiome, has been implicated in auto-immune, inflammatory, neurological diseases as well as in cancers. More recently it has also been shown to be associated with ocular diseases. In the present study, the association of gut microbiome dysbiosis with bacterial Keratitis, an inflammatory eye disease which significantly contributes to corneal blindness, was investigated. Bacterial and fungal gut microbiomes were analysed using fecal samples of healthy controls (HC, n=21) and bacterial Keratitis patients (BK, n=19). An increase in abundance of several anti-inflammatory organisms including Dialister, Megasphaera, Faecalibacterium, Lachnospira, Ruminococcus and Mitsuokella and members of Firmicutes, Veillonellaceae, Ruminococcaceae and Lachnospiraceae was observed in HC compared to BK patients in the bacterial microbiome. In the fungal microbiome, a decrease in the abundance of Mortierella, Rhizopus, Kluyveromyces, Embellisia and Haematonectria and an increase in the abundance of pathogenic fungi Aspergillus and Malassezia were observed in BK patients compared to HC. In addition, heatmaps, PCoA plots and inferred functional profiles also indicated significant variations between the HC and BK microbiomes, which strongly suggest dysbiosis in the gut microbiome of BK patients. This is the first study demonstrating the association of gut microbiome with the pathophysiology of BK and thus supports the gut-eye axis hypothesis. Considering that Keratitis affects about 1 million people annually across the globe, the data could be the basis for developing alternate strategies for treatment like use of probiotics or fecal transplantation to restore the healthy microbiome as a treatment protocol for Keratitis.