On the Development of the Japanese Eel, Anguilla japonica
On the Development of the Japanese Eel, Anguilla japonica
复制标题
论日本鳗鲡的开发
DOI：
10.2331/suisan.41.21
复制
发表时间：
1975
影响因子：
0.4
通讯作者：
S. Kasuga
中科院分区：
文献类型：
--
作者：
Kiichirô Yamamoto;K. Yamauchi;S. Kasuga
本研究所用的卵和乳分别取自经Synahorin处理成熟的雄鲑鱼和经垂体注射成熟的雌鲑鱼。采用干法受精，在23℃的正常海水中保存。受精卵直径约1.0mm，为远洋卵，呈典型的盘状卵裂。桑葚胚皮在受精后约3小时形成。约5小时后可见囊胚期卵，卵腔清晰。约9.5小时后发生原肠胚形成。当胚层覆盖1/3的蛋黄时，胚胎保护层就清晰了。此后，胚体开始形成，受精后24小时完成胚孔期。此时胚胎已分化出头体和10个小体。2) 25 h后胚胎形成眼囊和耳囊，28 h时胚胎形成心脏和30个小体。然后，心脏开始搏动，胚胎尾巴到达耳囊区。大约38小时后开始孵化。刚孵出的胚胎全长2.9毫米，卵黄囊较大。他们有40个人。很少的色素仅在胚胎的尾端区域被识别。
Eggs and milt used in this study were obtained from the male matured by the treatment with Synahorin and the female injected with salmon pituitary. And they were fertilized by the dry method and kept in normal sea water at 23°C. 1) Fertilized eggs of about 1.0mm in diameter were pelagic and showed a typical discoidal cleavage. Morula blastoderm were formed about 3 hours after insemination. Eggs in the blastula stage were seen about 5 hours later and showed a clear blastocoel. Gastrulation occurred about 91/2 hours after. When the blastoderm covered 1/3 yolk, an embryonic shield became clear. Thereafter, the embryonic body began to form, and the blastopore stage was completed 24 hours after fertiliza-tion. At that time the head and 10 somites were differentiated in the embryo. 2) Eye vesicles and ear vesicles were formed in the embryo 25 hours after, and the heart and 30 somites were discernible in the embryo 28 hours old. Then, the heart commenced pulse and an embryonic tail arrived at the ear vesicle region. Hatching began about 38 hours after. The embryos just after hatching measured 2.9mm in total length and had a large yolk sac. They had 40 somites. Very few pigments were recognizable only at the caudal region of the embryo.