Study on screening and antagonistic mechanisms of Bacillus amyloliquefaciens 54 against bacterial fruit blotch (BFB) caused by Acidovorax avenae subsp citrulli
Study on screening and antagonistic mechanisms of Bacillus amyloliquefaciens 54 against bacterial fruit blotch (BFB) caused by Acidovorax avenae subsp citrulli
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DOI：
10.1016/j.micres.2014.08.009
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发表时间：
2015-01-01
影响因子：
6.7
通讯作者：
Guo, Jian-Hua
中科院分区：
文献类型：
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作者：
Jiang, Chun-Hao;Wu, Fang;Guo, Jian-Hua
细菌性果斑病是葫芦科作物的严重威胁。它是由革兰氏阴性细菌Acidovorax avenue subsp.西瓜本研究从本实验室生防菌株库中筛选出的200株具有防治植物病害潜力的菌株，筛选出能有效防治细菌性果斑病的拮抗细菌。通过室内抑菌活性试验、温室试验和田间试验，筛选出5株对玉米赤霉病具有较强防治效果的菌株。5株细菌中有一株对细菌性痢疾的防治效果最好，命名为54。54的生防效果达60%以上。为了对该菌株进行鉴定，我们采用了一系列的方法对该菌株进行了鉴定，包括形态学分析、生理生化试验和生物分子测定。我们发现细菌54属于解淀粉芽孢杆菌。定殖试验结果表明，54号菌株定殖水平最高，其在叶片上的定殖密度可达105个菌落形成单位/克叶片组织。我们最近的研究结果表明，B.解淀粉菌54可提高植株体内速效N、P、K含量和叶绿素含量，促进植株生长。拮抗细菌54通过提高防御相关基因PR 1的表达水平和过氧化氢的积累，对BF B具有显著的控制作用。试验结果也验证了该菌的有效性。这也是B的首次报道。能够控制BFB的解淀粉菌株。(C)2014 Elsevier GmbH. All rights reserved.
Bacterial fruit blotch (BFB) was a serious threat to cucurbitaceae crops. It was caused by the gram-negative bacterium Acidovorax avenue subsp. citrulli. Two hundred strains, which have the potential in controlling plant diseases in our laboratory's biocontrol strain library, were employed to this research to screen some antagonistic bacteria, which can efficiently control bacterial fruit blotch disease. Based on the results of antagonistic activity experiments, greenhouse tests and field trials, 5 of the test strains have high abilities to control BFB. One of the 5 bacteria strains has the highest potential to control BFB named 54. The biocontrol efficacy of 54 was up to 60%. To characterize the strain, we used series of methods to evaluate the bacterium, including morphology analysis, physiological biochemical test and biomolecular assay. We found that the bacterium 54 belongs to the species Bacillus amyloliquefaciens. The colonization test results showed that 54 had the highest colonization levels, and the density of the strain on leaves was up 105 colony forming units (CFU) per gram of leaf tissue. Our recent results show that B. amyloliquefaciens 54 can promote the plant growth due to raised the contents of available N, P, K and the leaf chlorophyll. The antagonistic bacterium 54 can significantly control the BF B by increasing the expression level of defense-related gene PR1 and the accumulation the hydrogen peroxide in the plant. The results of trail experiment was also verified this efficient results of bacterium. This is also the first report of B. amyloliquefaciens strain that is able to control BFB. (C) 2014 Elsevier GmbH. All rights reserved.