Lipid Bilayer Disruption by Amphiphilic Janus Nanoparticles: The Role of Janus Balance
Lipid Bilayer Disruption by Amphiphilic Janus Nanoparticles: The Role of Janus Balance
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DOI：
10.1021/acs.langmuir.8b02298
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发表时间：
2018-10-16
期刊：
影响因子：
3.9
通讯作者：
Yu, Yan
中科院分区：
文献类型：
--
作者：
Lee, Kwahun;Yu, Yan
已知两亲性纳米颗粒会导致脂质双层的缺陷。然而，我们最近已经表明，当表面电荷和疏水基团在单个粒子的相反半球上空间分离时，它们的破坏作用显著增强。使用相同的两亲性阳离子/疏水Janus粒子体系，我们研究了粒子的亲水-亲油平衡(即Janus平衡)在它们与两性离子脂双层相互作用中的作用。我们发现，只有当颗粒的疏水一侧占据其表面的20%或更多时，Janus纳米颗粒才会在脂双层上诱导空洞。超过这一阈值，疏水表面积越大，颗粒对脂双层的吸引力越大，需要较低的颗粒浓度才能导致双层膜中的缺陷。这些结果建立了Janus颗粒表面疏水性覆盖率和颗粒对脂膜的破坏之间的定量关系。
Amphiphilic nanoparticles are known to cause defects in lipid bilayers. However, we have shown recently that their disruptive effects are significantly enhanced when surface charges and hydrophobic groups are spatially segregated on opposite hemispheres of a single particle. Using the same amphiphilic cationic/hydrophobic Janus particle system, here we investigate the role of the hydrophilic-lipophilic balance of the particles (namely the Janus balance) in their interaction with zwitterionic lipid bilayers. We show that Janus nanoparticles induce holes in lipid bilayers only when the hydrophobic side of particles occupies 20% or more of their surfaces. Beyond this threshold, the larger the hydrophobic surface area, the more attractive the particles are to lipid bilayers, and a lower particle concentration is needed for causing defects in the bilayers. The results establish a quantitative relationship between the surface coverage of hydrophobicity on the Janus particles and the particle-induced disruption to the lipid membranes.