mRNA and microRNA expression profiles of the NCI-60 integrated with drug activities.
mRNA and microRNA expression profiles of the NCI-60 integrated with drug activities.
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DOI：
10.1158/1535-7163.mct-09-0965
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发表时间：
2010-05
影响因子：
5.7
通讯作者：
Reinhold WC
中科院分区：
文献类型：
--
作者：
Liu H;D'Andrade P;Fulmer-Smentek S;Lorenzi P;Kohn KW;Weinstein JN;Pommier Y;Reinhold WC
作为聚焦分子分析系列的一部分，我们在这里展示了使用41，000探针Agilent全人类基因组寡核苷酸微阵列和15，000特征Agilent人类microRNA微阵列V2对NCI DTP药物筛选（NCI-60）的60个细胞系的mRNA和microRNA表达的新分析研究。测量了约21，000个基因和723个人类microRNA的表达水平。这些分析研究包括分别对6个和8个细胞系的mRNA和microRNA进行四倍技术重复，并对其余细胞系进行重复。由此产生的数据集可以在我们的CellMiner数据库中免费获得和在线搜索。结果表明，两种平台的重现性高，各种细胞类型之间具有基本的生物学相似性。mRNA和microRNA表达水平与我们先前发表的从NCI DTP药物筛选获得的1，429种抗癌活性化合物数据库整合。发现了大量mRNA和microRNA，主要与约1，300种药物（包括121种具有已知作用机制的药物）单向相关。这里提供的数据集将有助于识别可能相互影响和相互作用的mRNA、微小RNA和药物组。
As part of the Spotlight on Molecular Profiling series, we present here new profiling studies of mRNA and microRNA expression for the 60 cell lines of the NCI DTP drug screen (NCI-60) using the 41,000-probe Agilent Whole Human Genome Oligo Microarray and the 15,000-feature Agilent Human microRNA Microarray V2. The expression levels of ~21,000 genes and 723 human microRNAs were measured. These profiling studies include quadruplicate technical replicates for six and eight cell lines for mRNA and microRNA, respectively, and duplicates for the remaining cell lines. The resulting data sets are freely available and searchable online in our CellMiner database. The result indicates high reproducibility for both platforms and an essential biological similarity across the various cell types. The mRNA and microRNA expression levels were integrated with our previously published 1,429-compound database of anticancer activity obtained from the NCI DTP drug screen. Large blocks of both mRNAs and microRNAs were identified with predominately unidirectional correlations to ~1,300 drugs including 121 drugs with known mechanisms of action. The data sets presented here will facilitate the identification of groups of mRNAs, microRNAs and drugs that potentially affect and interact with one another.