Increased SKP2 and CKS1 gene expression contributes to the progression of human urothelial carcinoma
Increased SKP2 and CKS1 gene expression contributes to the progression of human urothelial carcinoma
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DOI：
10.1016/j.juro.2007.03.002
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发表时间：
2007-07-01
影响因子：
6.6
通讯作者：
Nakagawa, Masayuki
中科院分区：
文献类型：
--
作者：
Kawakami, Kazumori;Enokida, Hideki;Nakagawa, Masayuki
目的：SKP 2和CKS 1通过调节p27降解促进肿瘤的侵袭性行为。我们先前对人尿路上皮癌和正常尿路上皮的DNA微阵列分析显示，在尿路上皮癌中，SKP 2-p27相互作用相关基因中上调最高的2个基因是SKP 2（4.7倍）和CKS 1（2.2倍）。我们假设，SKP 2和CKS 1基因表达与尿路上皮癌的侵袭性和预后。材料和方法：共84例尿路上皮癌标本与膀胱（71）和上尿路（13）癌进行了实时逆转录聚合酶链反应和免疫组化检查。study.Results:实时荧光定量逆转录聚合酶链反应显示，SKP 2和CKS 1的平均mRNA表达水平与肿瘤分期显著相关，即浅表性尿路上皮癌与浸润性尿路上皮癌（SKP 2和CKS 1，p
Purpose: SKP2 and CKS1 promote aggressive tumor behavior via the regulation of p27 degradation. Our previous DNA microarray analysis of human urothelial carcinoma and normal urothelial epithelium showed that in urothelial carcinoma the 2 most highly up-regulated genes among SKP2-p27 interaction related genes are SKP2 (4.7-fold) and CKS1 (2.2-fold). We hypothesized that SKP2 and CKS1 gene expression is associated with urothelial carcinoma invasiveness and prognosis.Materials and Methods: A total of 84 urothelial carcinoma specimens from patients with bladder (71) and upper urinary tract (13) cancer were examined by real-time reverse transcriptase-polymerase chain reaction and immunohistochemical. study.Results: Real-time reverse transcriptase-polymerase chain reaction showed that the average mRNA expression level of SKP2 and CKS1 significantly correlated with tumor stage, that is superficial vs invasive urothelial carcinoma (SKP2 and CKS1, p