SDG714 Regulates Specific Gene Expression and Consequently Affects Plant Growth via H3K9 Dimethylation
SDG714 Regulates Specific Gene Expression and Consequently Affects Plant Growth via H3K9 Dimethylation
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SDG714 调节特定基因表达，从而通过 H3K9 二甲基化影响植物生长
DOI：
10.1111/j.1744-7909.2010.00927.x
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发表时间：
2010-04-01
影响因子：
11.4
通讯作者：
Dong, Ai-Wu
中科院分区：
文献类型：
--
作者：
Ding, Bo;Zhu, Yan;Dong, Ai-Wu
组蛋白赖氨酸甲基化参与了包括植物在内的所有真核生物基因表达的表观遗传调控。在这里，我们表明水稻SDG 714主要负责体内组蛋白H3 K9的二甲基化，而不是三甲基化。YFP-SDG 714在拟南芥中的过表达显著抑制植物生长，并且这种抑制与H3 K9二甲基化水平的提高相关。我们的微阵列结果显示，许多对植物生长和发育至关重要的基因在过表达YFP-SDG 714的转基因拟南芥中下调。通过染色质免疫沉淀分析，我们表明YFP-SDG 714靶向特定的染色质区域并二甲基化H3 K9，这与异染色质化和基因下调有关。最有趣的是，当YFP-SDG 714生产停止时，受抑制的植物可以部分恢复其生长，这表明YFP-SDG 714引起的基因表达扰动是可逆的。综上所述，我们的研究结果指出了SDG 714在H3 K9二甲基化，抑制基因表达和植物生长中的重要作用，并提供了一种通过SDG 714表达的开关来调节基因表达和植物发育的潜在方法。
Histone lysine methylation is known to be involved in the epigenetic regulation of gene expression in all eukaryotes including plants. Here we show that the rice SDG714 is primarily responsible for dimethylation but not trimethylation on histone H3K9 in vivo. Overexpression of YFP-SDG714 in Arabidopsis significantly inhibits plant growth and this inhibition is associated with an enhanced level of H3K9 dimethylation. Our microarray results show that many genes essential for the plant growth and development were downregulated in transgenic Arabidopsis plants overexpressing YFP-SDG714. By chromatin immunoprecipitation analysis, we show that YFP-SDG714 is targeted to specific chromatin regions and dimethylate the H3K9, which is linked with heterochromatinization and the downregulation of genes. Most interestingly, when YFP-SDG714 production is stopped, the inhibited plants can partially restore their growth, suggesting that the perturbation of gene expression caused by YFP-SDG714 is revertible. Taken together, our results point to an important role of SDG714 in H3K9 dimethylation, suppression of gene expression and plant growth, and provide a potential method to regulate gene expression and plant development by an on-off switch of SDG714 expression.