Effects of X-ray radiation on the rheologic properties of platelets and lymphocytes
Effects of X-ray radiation on the rheologic properties of platelets and lymphocytes
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DOI：
10.1046/j.1537-2995.2003.00360.x
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发表时间：
2003-04-01
期刊：
影响因子：
2.9
通讯作者：
Tran-Son-Tay, R
中科院分区：
文献类型：
--
作者：
Thomas, S;Bolch, W;Tran-Son-Tay, R
背景技术背景：血液往往是照射前输血严重免疫功能低下的患者，以防止潜在的致命并发症输血相关的GVHD.Study设计和方法：本研究评估的影响，X射线辐射对血小板和淋巴细胞的流变学，因为这些血细胞变形的能力是至关重要的，他们在整个微血管系统的流动。微管吸吮实验进行血小板和淋巴细胞暴露于X射线辐射剂量为0（对照），25，和50戈伊。结果：一个显着增加的杨氏弹性模量之间观察到对照血小板和照射血小板在25戈伊（p = 0.02）和50戈伊（p = 0.03）。在25 Gy照射的血小板中，细胞活化百分比显著增加（p = 0.008）。此外，25戈伊照射的淋巴细胞比对照组具有更高的粘度（p < 0.02）。50Gy照射后，血小板和淋巴细胞的变形性和活化性改变，可引起局部血流量减少和间歇性阻塞，从而引起微血管血流的改变。
BACKGROUND: Blood is often irradiated before transfusion for severely immunocompromised patients to prevent a potentially fatal complication of transfusion-associated GVHD.STUDY DESIGN AND METHODS: This study evaluates the effects of X-ray radiation on platelet and lymphocyte rheology because the ability of these blood cells to deform is vital to their flow throughout the microvascular system. Micropipette aspiration experiments were conducted on platelets and lymphocytes exposed to X-ray radiation doses of 0 (control), 25, and 50 Gy.RESULTS: A significant increase in the Young modulus of elasticity was observed between control platelets and irradiated platelets at 25 Gy (p = 0.02) and 50 Gy (p = 0.03). Percent cell activation increased significantly in 25 Gy-irradiated platelets (p = 0.008). In addition, lymphocytes irradiated at 25 Gy have a higher viscosity than controls (p < 0.02). A significantly larger number of activated cells is found in the 50 Gy-irradiated lymphocyte population (p < 0.04).CONCLUSION: The changes in the deformability and activation of irradiated platelets and lymphocytes may reduce local blood flow and lead to intermittent blockage, which may cause a change of blood flow in microvasculatures.