Avian Influenza Virus Detection by Optimized Peptide Termination on a Boron-Doped Diamond Electrode
Avian Influenza Virus Detection by Optimized Peptide Termination on a Boron-Doped Diamond Electrode
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DOI：
10.1021/acssensors.9b02126
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发表时间：
2020-02-01
期刊：
影响因子：
8.9
通讯作者：
Sato, Toshinori
中科院分区：
文献类型：
--
作者：
Matsubara, Teruhiko;Ujie, Michiko;Sato, Toshinori
开发简单、高灵敏度的检测方法对于传染病的诊断和监测至关重要。此前，我们使用掺硼金刚石电极构建了一种灵敏的生物传感器，用于检测病理性人类流感病毒，该电极末端是唾液酸寡糖受体模拟肽，可以与病毒感染中涉及的血凝素结合。禽病毒引起的流感在人类中的传播已成为主要的公共卫生问题，迫切需要检测禽病毒的方法。在此，电极上终止的肽密度和树枝状聚合物的生成改变了病毒与电极表面结合的效率，从而显着增强了电化学阻抗谱测量的电荷转移电阻。肽端接电极表现出出色的检测限，对季节性 H1N1 和 H3N2 病毒的检测限小于 1 个空斑形成单位。此外，改进的电极可检测从 H5N3、H7NI 和 H9N2 中分离出的禽类病毒，显示出检测甲型流感病毒所有亚型（包括新亚型）的潜力。基于肽的电化学结构为超灵敏检测病原微生物的生物传感器提供了一种有前途的方法。
The development of a simple detection method with high sensitivity is essential for the diagnosis and surveillance of infectious diseases. Previously, we constructed a sensitive biosensor for the detection of pathological human influenza viruses using a boron-doped diamond electrode terminated with a sialyloligosaccharide receptor-mimic peptide that could bind to hemagglutinins involved in viral infection. Circulation of influenza induced by the avian virus in humans has become a major public health concern, and methods for the detection of avian viruses are urgently needed. Here, peptide density and dendrimer generation terminated on the electrode altered the efficiency of viral binding to the electrode surface, thus significantly enhancing charge-transfer resistance measured by electrochemical impedance spectroscopy. The peptide-terminated electrodes exhibited an excellent detection limit of less than one plaque-forming unit of seasonal H1N1 and H3N2 viruses. Furthermore, the improved electrode was detectable for avian viruses isolated from H5N3, H7NI, and H9N2, showing the potential for the detection of all subtypes of influenza A virus, including new subtypes. The peptide-based electrochemical architecture provided a promising approach to biosensors for ultrasensitive detection of pathogenic microorganisms.